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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Garcia, J. F. Moreno, V. R. Grisolia, A. B. Adania, C. H. Campagnari, F. Milazzotto, M. P. Souza, E. B. |
| Description | Country affiliation: Brazil Responsible library: BR1.1 Author Affiliation: Grisolia, A. B ( UNESP. Instituto de Biociências. Departamento de Genética. Botucatu. BR); Adania, C. H ( Associação Mata Ciliar. Jundiaí. BR); Souza, E. B ( UNESP. Faculdade de Ciências e Letras. Assis. BR); Garcia, J. F ( UNESP. LBBMA. Departamento de Apoio, Produção e Saúde Animal. Araçatuba. BR); Milazzotto, M. P ( UNESP. Instituto de Biociências. Departamento de Genética. Botucatu. BR); Campagnari, F ( UNESP. Instituto de Biociências. Departamento de Genética. Botucatu. BR); Moreno, V. R ( UNESP. Instituto de Biociências. Departamento de Genética. Botucatu. BR) |
| Abstract | The microsatellite loci FCA045, FCA077, FCA008, and FCA096 are highly variable molecular markers which were used to determine the genetic diversity in 148 captive Leopardus sp. The PCR-amplified products of microsatellite loci were characterized in ABI Prism 310 Genetic Analyzer. Allele numbers, heterozygosity, polymorphism information content, exclusive allele number, and shared alleles were calculated. Sixty-five alleles were found and their sizes ranged from 116 to 216 bp in four microsatellite loci. The heterozygosity ranged from 0.36 to 0.81 in Leopardus pardalis, 0.57 to 0.67 in L. tigrinus and 0.80 to 0.92 in L. wiedii. The polymorphism information content was from 0.80 to 0.88 in L. pardalis, 0.76 to 0.88 in L. tigrinus and 0.77 to 0.90 in L. wiedii. The margay (L. wiedii) showed the highest index of polymorphism among the three species in this study. These results imply that microsatellite DNA markers can help in the study of the genetic diversity of Leopardus specimens. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 2 |
| Volume Number | 6 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2007-01-01 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Animals Felidae Genetics Microsatellite Repeats Genetic Variation Alleles Gene Frequency Heterozygote Models, Genetic Polymorphism, Genetic Species Specificity Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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