NDLI: RT-qPCR assay on the vitamin D receptor gene in type 2 diabetes and hypertension patients in Turkey.

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RT-qPCR assay on the vitamin D receptor gene in type 2 diabetes and hypertension patients in Turkey.

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RT-qPCR assay on the vitamin D receptor gene in type 2 diabetes and hypertension patients in Turkey.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Maltas, E. Vural, H. C.
Spatial Coverage	Turkey
Description	Country affiliation: Turkey Author Affiliation: Vural HC ( Department of Biology, Faculty of Science, Selcuk University, Konya, Turkey.)
Abstract	RT-qPCR was used to analyze the vitamin D receptor (VDR) gene TaqI polymorphism in 100 Turkish patients with type 2 diabetes mellitus (T2DM) and hypertension compared with 100 healthy subjects, to determine whether VDR could be considered as one of the susceptibility genes for T2DM and hypertension. Genotyping was done with PCR, followed by melting curve analysis with specific fluorescent hybridization probes. The results showed that distributions for TT, Tt and tt genotypes were 51, 46 and 3% in the patient group, and 35, 49 and 16% in the control group, respectively. The frequency of the T allele in patients was also significantly higher than that in controls. Based on the results, the relationship between the VDR gene TaqI polymorphism and T2DM patients in the Turkish population was compared. In terms of the genotype distributions and allele frequencies of the VDR gene TaqI polymorphism, there was no statistically significant difference (P > 0.05) between the T2DM and hypertension patients and controls. Application of RT-qPCR method enabled us to assess the prevalence of the VDR gene TaqI polymorphism and its association with type 2 diabetes and hypertension.
e-ISSN	16765680
Journal	Genetics and Molecular Research
Issue Number	1
Volume Number	11
Language	English
Publisher	Fundação de Pesquisas Científicas de Ribeirão Preto
Publisher Date	2012-03-14
Publisher Place	Brazil
Access Restriction	Open
Subject Keyword	Diabetes Mellitus, Type 2 Genetics Hypertension Receptors, Calcitriol Gene Frequency Genetic Predisposition To Disease Genotype Polymorphism, Genetic Real-time Polymerase Chain Reaction Turkey Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics
Content Type	Text
Resource Type	Article

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