NDLI: Establishment of an in vitro regeneration system for genetic transformation of selected sugarcane genotypes.

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Establishment of an in vitro regeneration system for genetic transformation of selected sugarcane genotypes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Rana, I. A. Saleem, M. Ijaz, S. Khan, I. A.
Description	Country affiliation: Pakistan Author Affiliation: Ijaz S ( Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture Faisalabad, Pakistan. siddraijazkhan@yahoo.com)
Abstract	A good culture system provides considerable quantities of highly regenerable target tissues. Embryogenic callus cultures are ideal for micro-projectile-mediated transformation, because regenerable cells are not very stable. Effective exploitation of genetic transformation requires good regeneration systems. We selected three sugarcane genotypes for the establishment and optimization of good in vitro regeneration systems, viz., S-2003-us-359, S-2006-sp-30, and S-2003-us-165. Three callus induction media were investigated. These media were composed of Murashige and Skoog (MS) medium salt plus 1, 2, and 3 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D). Medium with 3 mg/L 2,4-D gave the greatest mass of embryogenic calli. The calli produced on the three callus induction media were transferred to 18 types of regeneration media (RM1-RM18). They varied with respect to plant growth regulators and sucrose levels but the basal medium was MS. Two levels of sucrose (30 and 40 g/L), three levels of 2,4-D (0.1, 0.25, 0.5 mg/L) and three levels of 6-benzylaminopurine (0, 0.25 and 0.5 mg/L) were studied in the regeneration media. The effects of callus age on regeneration were evaluated by transferring the calli to regeneration media after 15, 21, 28, and 35 days of culture. The 21-day-old callus of the genotype S-2003-us-359 on RM3 yielded the largest number of plants and was selected as the best for transformation. Six RAPD DNA primers were used to check genetic stability; this medium did not affect the sugarcane genomes.
e-ISSN	16765680
Journal	Genetics and Molecular Research
Issue Number	1
Volume Number	11
Language	English
Publisher	Fundação de Pesquisas Científicas de Ribeirão Preto
Publisher Date	2012-03-06
Publisher Place	Brazil
Access Restriction	Open
Subject Keyword	Genotype Regeneration Saccharum Genetics Transformation, Genetic Culture Media Genomic Instability Plant Roots Growth & Development Plant Shoots Plants, Genetically Modified Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics
Content Type	Text
Resource Type	Article

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