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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Duran, Robert Bonin, Patricia Jezequel, Ronan Militon, Cecile Gilbert, Franck Dubosc, Karine Gassie, Claire Terrisse, Fanny Abella, Justine Cravo-Laureau, Cristiana Cuny, Philippe Cagnon, Christine Michotey, Valérie |
| Description | Country affiliation: France Author Affiliation: Duran R ( Equipe Environnement et Microbiologie, MELODY Group, Université de Pau et des Pays de l'Adour, IPREM UMR CNRS 5254, BP 1155, 64013, Pau Cedex, France. Robert.duran@univ-pau.fr.); Bonin P ( Université de Pau et des Pays de l'Adour, Bâtiment IBEAS, BP 1155, 64013, Pau Cedex, France. Robert.duran@univ-pau.fr.); Jezequel R ( Aix Marseille Université, CNRS, IRD, MIO UM 110, 13288, Marseille, France.); Dubosc K ( Centre de Documentation, de Recherche et d'Expérimentations sur les pollutions accidentelles des Eaux, 715 rue Alain Colas, CS 41836, Brest, France.); Gassie C ( Centre de Documentation, de Recherche et d'Expérimentations sur les pollutions accidentelles des Eaux, 715 rue Alain Colas, CS 41836, Brest, France.); Terrisse F ( Equipe Environnement et Microbiologie, MELODY Group, Université de Pau et des Pays de l'Adour, IPREM UMR CNRS 5254, BP 1155, 64013, Pau Cedex, France.); Abella J ( Equipe Environnement et Microbiologie, MELODY Group, Université de Pau et des Pays de l'Adour, IPREM UMR CNRS 5254, BP 1155, 64013, Pau Cedex, France.); Cagnon C ( Equipe Environnement et Microbiologie, MELODY Group, Université de Pau et des Pays de l'Adour, IPREM UMR CNRS 5254, BP 1155, 64013, Pau Cedex, France.); Militon C ( Equipe Environnement et Microbiologie, MELODY Group, Université de Pau et des Pays de l'Adour, IPREM UMR CNRS 5254, BP 1155, 64013, Pau Cedex, France.); Michotey V ( Aix Marseille Université, CNRS, IRD, MIO UM 110, 13288, Marseille, France.); Gilbert F ( Aix Marseille Université, CNRS, IRD, MIO UM 110, 13288, Marseille, France.); Cuny P ( Université de Toulouse); Cravo-Laureau C ( CNRS) |
| Abstract | The present study aimed to examine whether the physical reworking of sediments by harrowing would be suitable for favouring the hydrocarbon degradation in coastal marine sediments. Mudflat sediments were maintained in mesocosms under conditions as closer as possible to those prevailing in natural environments with tidal cycles. Sediments were contaminated with Ural blend crude oil, and in half of them, harrowing treatment was applied in order to mimic physical reworking of surface sediments. Hydrocarbon distribution within the sediment and its removal was followed during 286 days. The harrowing treatment allowed hydrocarbon compounds to penetrate the first 6 cm of the sediments, and biodegradation indexes (such as n-C18/phytane) indicated that biodegradation started 90 days before that observed in untreated control mesocosms. However, the harrowing treatment had a severe impact on benthic organisms reducing drastically the macrofaunal abundance and diversity. In the harrowing-treated mesocosms, the bacterial abundance, determined by 16S rRNA gene Q-PCR, was slightly increased; and terminal restriction fragment length polymorphism (T-RFLP) analyses of 16S rRNA genes showed distinct and specific bacterial community structure. Co-occurrence network and canonical correspondence analyses (CCA) based on T-RFLP data indicated the main correlations between bacterial operational taxonomic units (OTUs) as well as the associations between OTUs and hydrocarbon compound contents further supported by clustered correlation (ClusCor) analysis. The analyses highlighted the OTUs constituting the network structural bases involved in hydrocarbon degradation. Negative correlations indicated the possible shifts in bacterial communities that occurred during the ecological succession. |
| ISSN | 09441344 |
| Issue Number | 20 |
| Volume Number | 22 |
| e-ISSN | 16147499 |
| Journal | Environmental Science and Pollution Research |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2015-10-01 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Bacteria Metabolism Geologic Sediments Microbiology Hydrocarbons Petroleum Water Pollutants, Chemical Genetics Isolation & Purification Biodegradation, Environmental Dna, Bacterial Chemistry Analysis Phylogeny Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Rna, Ribosomal, 16s Sequence Analysis, Dna Journal Article Research Support, Non-u.s. Gov't Discipline Environmental Science Discipline Environmental Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Environmental Chemistry Health, Toxicology and Mutagenesis Pollution Medicine |
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