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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Huang, Min Joon Lim, Eu-Jin Andy Tay, Guang Kai Ignatius Liu, Edison T. Zhang, Guo-Jun Luo, Zhan Hong Henry Thomsen, Jane S. |
| Description | Country affiliation: Singapore Author Affiliation: Zhang GJ ( Institute of Microelectronics, A*STAR (Agency for Science, Technology and Research), 11 Science Park Road, Singapore Science Park II, Singapore 117685, Singapore. zhanggj@ime.a-star.edu.sg) |
| Abstract | To thoroughly understand the role that estrogen receptors partake in regulation of gene expression, characterization of estrogen receptors (ERs) and estrogen-response elements (EREs) interactions is essential. In the work, we present a highly sensitive and reusable silicon nanowire (SiNW) biosensor to study the interactions between human ER proteins (ER, and ß subtypes) and EREs (dsDNA). The proteins were covalently immobilized on the SiNW surface. Various EREs including wild-type, mutant and scrambled DNA sequences were then applied to the protein-functionalized SiNW surface. Due to negatively charged dsDNA, binding of the EREs to the ERs on the n-type SiNW biosensor leads to the accumulation of negative charges on the surface, thereby inducing increase in resistance. The results show that the specificity of the ERE-ER binding is higher than that of the ERE-ERß binding, what is more, the mutant ERE reduces the binding affinity for both ER and ERß. By applying various concentrations of wild-type ERE to the bound ER , a very low concentration of 10 fM wild-type ERE was found to be able to bind to the ER . The reversible association and dissociation between ER and wt-ERE was achieved, pointing to a reusable biosensor for protein-DNA binding. Through the study, we have established the SiNW biosensor as a promising method in providing comprehensive study for hormone receptor-response element interactions. |
| ISSN | 09565663 |
| Issue Number | 2 |
| Volume Number | 26 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-10-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Instrumentation Conductometry Dna Chemistry Nanotubes Protein Interaction Mapping Receptors, Estrogen Silicon Equipment Design Equipment Failure Analysis Equipment Reuse Nanotechnology Ultrastructure Response Elements Sensitivity And Specificity Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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