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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Luo, Yilun Hochhalter, David Alocilja, Evangelyn C. Drzal, Lawrence T. Nartker, Steven Setterington, Emma Wiederoder, Sara Wiederoder, Michael Miller, Hanna |
| Description | Country affiliation: United States Author Affiliation: Luo Y ( Department of Biosystems and Agricultural Engineering, Michigan State University, Lansing, MI 48824, USA.) |
| Abstract | Electrospinning is a versatile and cost effective method to fabricate biocompatible nanofibrous materials. The novel nanostructure significantly increases the surface area and mass transfer rate, which improves the biochemical binding effect and sensor signal to noise ratio. This paper presents the electrospinning method of nitrocellulose nanofibrous membrane and its antibody functionalization for application of bacterial and viral pathogen detection. The capillary action of the nanofibrous membrane is further enhanced using oxygen plasma treatment. An electrospun biosensor is designed based on capillary separation and conductometric immunoassay. The silver electrode is fabricated using spray deposition method which is non-invasive for the electrospun nanofibers. The surface functionalization and sensor assembly process retain the unique fiber morphology. The antibody attachment and pathogen binding effect is verified using the confocal laser scanning microscope (CLSM) and scanning electronic microscope (SEM). The electrospun biosensor exhibits linear response to both microbial samples, Escherichia coli O157:H7 and bovine viral diarrhea virus (BVDV) sample. The detection time of the biosensor is 8 min, and the detection limit is 61 CFU/mL and 10(3)CCID/mL for bacterial and viral samples, respectively. With the advantage of efficient antibody functionalization, excellent capillary capability, and relatively low cost, the electrospinning process and surface functionalization method can be implemented to produce nanofibrous capture membrane for different immuno-detection applications. |
| ISSN | 09565663 |
| Issue Number | 4 |
| Volume Number | 26 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-12-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Instrumentation Methods Diarrhea Viruses, Bovine Viral Isolation & Purification Escherichia Coli O157 Nanofibers Animals Antibodies, Bacterial Antibodies, Immobilized Antibodies, Viral Cattle Collodion Chemistry Immunology Pathogenicity Electrochemical Techniques Mice Microscopy, Confocal Microscopy, Electron, Scanning Ultrastructure Nanotechnology Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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