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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Mandon, Céline A. Blum, Loïc J. Heyries, Kevin A. Marquette, Christophe A. |
| Description | Country affiliation: France Author Affiliation: Mandon CA ( Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Equipe Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires, Université Lyon 1-CNRS 5246 ICBMS, Bâtiment CPE 43, bd du 11 novembre 1918, 69622 Villeurbanne, Cedex, France.) |
| Abstract | A new approach for the rapid production of microfluidic chips integrating protein spots is described. The technology, called 'Print-n-Shrink', is based on the screen-printing of a microfluidic design (using a dielectric ink) onto Polyshrink™ polystyrene sheets. The initial printing which have a minimum size of 15 µm (height)×230 µm (width) was thermally treated (30s, 163°C) to shrink and generate features of 85 µm (height)×100 µm (width). Protein spots were also demonstrated to be shrinkable and arrays of 50 µm-size spots with density up to 6400 spots/cm(2) were achieved. Proteins such as monoclonal antibodies or cellular adhesion proteins were thus spotted onto the Polyshrink™ sheets and shrunk together with the microfluidic design, creating complete biochips integrating both complex microfluidic designs and protein spots for bioanalytical applications. These shrunk spots were shown to host enough active proteins to enable the achievement of both sensitive sandwich immunoassays (Brain Natriuretic Peptide, C-Reactive Protein and c-Troponin I) and localized cell culture. |
| ISSN | 09565663 |
| Issue Number | 4 |
| Volume Number | 26 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-12-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Microfluidic Analytical Techniques Instrumentation Protein Array Analysis Animals Antibodies, Monoclonal Cattle Equipment Design Hela Cells Humans Immunoassay Ink Luminescent Measurements Microelectrodes Polystyrenes Printing Proteins Serum Albumin, Bovine Journal Article Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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