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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Wu, Chi-Man Lawrence Kong, S. K. Wu, Shu-Yuen Ho, Ho-Pui Ng, Siu-Pang |
| Description | Author Affiliation: Ng SP ( Department of Physics and Materials Science, City University of Hong Kong, Hong Kong SAR, PR China.) |
| Abstract | We introduce a novel wide dynamic range phase-sensitive surface plasmon resonance (SPR) biosensor based on differential spectral interferometry. Superseding conventional spectroscopic approach where only the SPR dip is monitored, our system acquires the spectral phase information of the entire electromagnetic field that undergoes SPR transformation. Since the SPR-induced phase change is highly wavelength specific with fixed incident angle, ultra-high sensitivity achievable through phase-sensitive detection, as reported herein, is maintained continuously across the spectral domain in response to refractive index changes. Our system has demonstrated a detection limit of 2.2×10(-7) in terms of refractive index unit (RIU) using standard single-layer gold surface. In terms of biosensing performance, the estimated detection sensitivity obtained from bovine serum albumin (BSA) antibody-antigen binding experiments is 0.5 ng ml(-1). |
| ISSN | 09565663 |
| Issue Number | 4 |
| Volume Number | 26 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-12-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Methods Dielectric Spectroscopy Interferometry Surface Plasmon Resonance Animals Antigen-antibody Reactions Instrumentation Statistics & Numerical Data Cattle Gold In Vitro Techniques Limit Of Detection Refractometry Serum Albumin, Bovine Immunology Metabolism Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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