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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Homola, Jirí Bocková, Markéta Piliarik, Marek |
| Description | Country affiliation: Czech Republic Author Affiliation: Piliarik M ( Institute of Photonics and Electronics AS CR v.v.i., Chaberská 57, CZ-18251 Prague 8, Czech Republic.) |
| Abstract | Surface plasmon resonance (SPR) biosensor for high-throughput screening of protein biomarkers in diluted blood plasma is reported. The biosensor combines a high-resolution SPR imaging sensor and a high-density protein array with low-fouling background. The SPR imaging sensor utilizes polarization contrast and advanced referencing and provides a total of 120 sensing areas (each 200 µm×150 µm). Antibodies are immobilized on the sensing areas via hybridization of antibody-oligonucleotide conjugates to thiolated complementary oligonucleotides microspotted on the sensor surface (DNA-directed immobilization). A low-fouling background is achieved by covalent immobilization of bovine serum albumin to carboxyl-terminated thiols filling the areas among the thiolated oligonucleotides and outside the sensing areas. The biosensor was evaluated for detection of protein biomarkers relevant to cancer diagnostics--human chorionic gonadotropin (hCG) and activated leukocyte cell adhesion molecule (ALCAM) both in buffer and in 10% blood plasma. Limits of detection as low as 45 ng/mL (ALCAM) and 100 ng/mL (hCG) were achieved in blood plasma samples. |
| ISSN | 09565663 |
| Issue Number | 4 |
| Volume Number | 26 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-12-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biological Markers Blood Blood Proteins Analysis Surface Plasmon Resonance Instrumentation Animals Antibodies, Immobilized Antigens, Cd Tumor Markers, Biological Cattle Cell Adhesion Molecules, Neuronal Chorionic Gonadotropin Equipment Design Fetal Proteins Humans Immobilized Proteins Limit Of Detection Neoplasms Diagnosis Oligonucleotides Refractometry Serum Albumin, Bovine Methods Statistics & Numerical Data Thionucleotides Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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