NDLI: Peptide binding to ochratoxin A mycotoxin: a new approach in conception of biosensors.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	De Waard, M. Gonzalez, C. Sabatier, J. M. Hassine, A. Ibn Hadj Bazin, I. Andreotti, N.
Description	Country affiliation: France Author Affiliation: Bazin I ( Ecole des Mines d'Ales, 6 avenue de Claviers 30319 Ales cedex, France. ingrid.bazin@mines-ales.fr)
Abstract	Ochratoxin A (OTA) is a widespread and abundant natural carcinogenic mycotoxin produced by several species of Aspergillus and Penicillium fungi. Due to the ubiquitous presence of these fungi in food and potential risk for human health, a rapid and sensitive in vitro detection assay is required. Analytical methods for OTA detection/identification are generally based on liquid-liquid extraction, clean-up using an immunoaffinity column (IAC), and identification by reversed-phase high pressure liquid chromatography with fluorescence detection (HPLC-FLD). However, IACs are costly and have a short lifespan. Therefore, an interesting approach would appear to be the design and chemical synthesis of a mimotope peptide simulating mycotoxin-specific antibodies. We have developed a promising alternative method that is based on the use of peptides which are able to bind to specific chemical functions and/or molecular structures. Accordingly, a number of peptides (derived from the structures of major redox proteins) were selected and produced by chemical solid phase syntheses. The ability of such peptides to bind to ochratoxin A was evaluated by HPLC. The peptide NF04 (structurally derived from an oxidoreductase enzyme), which was found to be the sole potently reactive compound among tested molecules, was further evaluated in a peptide-based enzyme-linked immunosorbent assay (peptide-based ELISA), thus confirming its specific interaction with ochratoxin A.
ISSN	09565663
Issue Number	1
Volume Number	40
e-ISSN	18734235
Journal	Biosensors and Bioelectronics
Language	English
Publisher	Elsevier
Publisher Date	2013-02-15
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biosensing Techniques Instrumentation Enzyme-linked Immunosorbent Assay Ochratoxins Analysis Chemistry Peptides Protein Interaction Mapping Equipment Design Equipment Failure Analysis Mycotoxins Reproducibility Of Results Sensitivity And Specificity Journal Article Discipline Biotechnology
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry

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