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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zou, Xiaoyong Fan, Suhua Zhu, Wenyuan Wu, Hai Dai, Zong |
| Description | Author Affiliation: Wu H ( School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.) |
| Abstract | An electrochemical approach based on protein controllable unfolding was developed and applied in combination with in-situ reaction in order to investigate the electrocatalytic pathway for hemoglobin (Hb) toward nitric oxide (NO). Hb was entrapped in a dimethyldidodecylammonium bromide (DDAB) film modified glassy carbon electrode (DDAB/Hb/GCE). Two typical denaturants of acid and urea were synergistically utilized to control the incorporated Hb to a most unfolded state without losing heme groups. Under optimal conditions, the unfolded DDAB/Hb/GCE exhibited accelerated direct electron transfer. The sensitivities for the detection of ascorbic acid (AA), NaNO(2) and NO were improved as 3, 10 and 12 times higher than those on the native DDAB/Hb/GCE, and the limits of detection (LODs) for AA, NaNO(2) and NO were down to 0.33, 0.83 and 0.063 µM, respectively. The unfolded DDAB/Hb/GCE was further applied for the investigation of Hb-NO interaction in NaNO(2) solution. With successive additions of AA, NO was generated in situ on DDAB/Hb/GCE. A new reduction peak of the intermediate HbFe(II)-HN(2)O(2) was successfully revealed near -0.65 V. The whole electrocatalytic mechanism was proposed and verified by density functional theory. The method can be a promising platform for facile study of the interaction between NO and heme proteins. |
| ISSN | 09565663 |
| Volume Number | 41 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-03-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Instrumentation Conductometry Hemoglobins Chemistry Nitric Oxide Protein Interaction Mapping Binding Sites Catalysis Equipment Design Equipment Failure Analysis Protein Binding Protein Folding Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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