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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Wang, Jingpeng Thomas, Dan F. Ahmadalinezhad, Asieh Chen, Aicheng Kafi, A. K. M. |
| Description | Country affiliation: Canada Author Affiliation: Kafi AK ( Department of Chemistry, Lakehead University, Thunder Bay, Ontario P7B 5E1, Canada.) |
| Abstract | In this work, we have directly grown three-dimensional nanoporous Au networks onto a Ti substrate using the hydrothermal technique. This newly designed material with a large surface area was used as a supporting matrix for immobilizing a redox protein, hemoglobin (Hb), to develop a high-performance hydrogen peroxide biosensor. Scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), and energy-dispersive X-ray (EDX) spectroscopy were employed to characterize the morphology and composition of the fabricated nanoporous Au network. Cyclic voltammetry (CV) and amperometry were used to study and to optimize the performance of the fabricated electrochemical biosensor. Our CV studies show the direct electron transfer of Hb immobilized on the nanoporous Au network. In addition, amperometric H(2)O(2) sensing experiments revealed that the nanoporous Au-network based biosensor exhibits fast response, long linearity, a low detection limit, high stability and very good reproducibility. Under the optimized conditions, the linearity of the developed biosensor for the detection of H(2)O(2) spans from 5 x 10(-8) to 2 x 10(-4)M with a detection limit of 2 x 10(-8)M (based on S/N=3). |
| ISSN | 09565663 |
| Issue Number | 11 |
| Volume Number | 25 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-07-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Instrumentation Conductometry Gold Chemistry Hemoglobins Hydrogen Peroxide Analysis Nanostructures Nanotechnology Adsorption Equipment Design Equipment Failure Analysis Ultrastructure Porosity Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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