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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Galan, Carlos A. Hernandez, Prisciliano Castrillejo, Yolanda Barrado, Enrique Rodriguez, Jose A. |
| Description | Country affiliation: Mexico Author Affiliation: Hernandez P ( Centro de Investigaciones Quimicas, Universidad Autonoma el Estado de Hidalgo, Carr. Pachuca-Tulancingo km 4.5, C.P. 42076, Pachuca, Hidalgo, Mexico.) |
| Abstract | An amperometric flow system for glucose determination in blood serum samples after enzymatic reaction with glucose oxidase immobilized on magnetite covered with silica gel modified propylamine is described. The solid was magnetically retained on a mini-column and placed into the flow injection system preceding the amperometric detector using a modified screen printed electrode with [Fe(tris(3,5-dimetyl-1-pyrazolyl)borate)(2)](+)[FeCl(4)](-). The variables involved in the system such as flow rate, enzyme concentration, injection volume and reaction coil length were evaluated using a Taguchi parameter design. Under optimal conditions, the calibration curve of glucose sample was linear between 0.24 and 6.00 mM, and with a limit of detection of 0.08 mM. The repeatability for a 4.0mM glucose solution was 1.0%.The method was validated by comparing the obtained results to those provided by the enzymatic spectrophotometric method; no significant differences were observed. |
| ISSN | 09565663 |
| Volume Number | 41 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-03-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Instrumentation Blood Glucose Analysis Conductometry Glucose Oxidase Chemistry Immunomagnetic Separation Microfluidic Analytical Techniques Enzymes, Immobilized Equipment Design Equipment Failure Analysis Humans Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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