NDLI: Bio-mimetically synthesized Ag@BSA microspheres as a novel electrochemical biosensing interface for sensitive detection of tumor cells.

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Bio-mimetically synthesized Ag@BSA microspheres as a novel electrochemical biosensing interface for sensitive detection of tumor cells.

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Bio-mimetically synthesized Ag@BSA microspheres as a novel electrochemical biosensing interface for sensitive detection of tumor cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wang, Xiansong Jia, Nengqin Chen, Di Cui, Daxiang Hu, Chenyi Wang, Zhihua Yang, Da-Peng Huang, Peng Yang, Mo
Description	Country affiliation: China Author Affiliation: Hu C ( The Education Ministry Key Laboratory of Resource Chemistry, Department of Chemistry, Shanghai Normal University, 100 Guilin Road, Shanghai 200240, China.)
Abstract	The use of a novel cytosensor, comprised of bio-mimetically synthesized Ag@BSA composite microspheres, for the detection of KB cells (a model system) is described. The Ag@BSA composite microspheres were immobilized on Au electrodes via Au-thiol bonds. Scanning electron microscopy (SEM), atomic force microscopy (AFM) and transmission electron microscopy (TEM) images revealed that the Ag@BSA were well-dispersed microspheres with an average diameter of 500 nm, including the monolayer of BSA. The immobilization of Ag@BSA composite microspheres onto Au electrodes is thought to increase the electrode surface area and accelerate the electron transfer rate while providing a highly stable matrix for the convenient conjugation of target molecules (such as folic acid) and the prolonged incubation of cells. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) studies showed that the fabricated cytosensor was able to detect KB cells ranging from 6.0×10(1) to 1.2×10(8) cells mL(-1) with a lower detection limit of 20 cells mL(-1). Due to its facile synthesis, high stability and reproducibility and cytocompatibility, the novel cytosensor described here could find multifarious uses in applications, such as cancer diagnosis, drug screening and cell adhesion studies.
ISSN	09565663
Volume Number	41
e-ISSN	18734235
Journal	Biosensors and Bioelectronics
Language	English
Publisher	Elsevier
Publisher Date	2013-03-15
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biomimetic Materials Chemical Synthesis Biosensing Techniques Instrumentation Conductometry Cytodiagnosis Neoplasms, Experimental Diagnosis Serum Albumin, Bovine Chemistry Silver Methods Equipment Design Equipment Failure Analysis Humans Kb Cells Microspheres Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry

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