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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Trono, C. Biswas, P. Baldini, F. Tombelli, S. Bandyopadhyay, S. Giannetti, A. Chiavaioli, F. Basumallick, N. Dasgupta, K. |
| Description | Country affiliation: Italy Author Affiliation: Chiavaioli F ( Institute of Applied Physics 'Nello Carrara', National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italy.); Biswas P ( Central Glass and Ceramic Research Institute, Council of Scientific and Industrial Research, 196 Raja S C Mullick Road, Kolkata 700032, India. Electronic address: palas@cgcri.res.in.); Trono C ( Institute of Applied Physics 'Nello Carrara', National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italy. Electronic address: c.trono@ifac.cnr.it.); Bandyopadhyay S ( Central Glass and Ceramic Research Institute, Council of Scientific and Industrial Research, 196 Raja S C Mullick Road, Kolkata 700032, India.); Giannetti A ( Institute of Applied Physics 'Nello Carrara', National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italy.); Tombelli S ( Institute of Applied Physics 'Nello Carrara', National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italy.); Basumallick N ( Central Glass and Ceramic Research Institute, Council of Scientific and Industrial Research, 196 Raja S C Mullick Road, Kolkata 700032, India.); Dasgupta K ( Central Glass and Ceramic Research Institute, Council of Scientific and Industrial Research, 196 Raja S C Mullick Road, Kolkata 700032, India.); Baldini F ( Institute of Applied Physics 'Nello Carrara', National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italy.) |
| Abstract | Long period fiber gratings have been effectively used in the field of biochemical sensing since a few years. Compared to other well-known label-free optical approaches, long period gratings (LPGs) take advantage of the typical peculiarity of optical fibers. Coupling the propagating core mode with a high-order cladding mode near its turn-around point (TAP) was the strategy adopted to achieve good performances without additional coatings, except for the sensing and selective biolayer deposited on the fiber. Both the modeling and manufacturing of TAP LPGs were discussed. After the functionalization of the fiber surface with the deposition of a Eudragit L100 copolymer layer followed by immunoglobulin G (IgG) covalent immobilization, an IgG/anti-IgG bioassay was implemented along the grating region and the kinetics of antibody/antigen interaction was analyzed. A quantitative comparison between a TAP LPG and a non-TAP LPG was carried out to highlight the improvement of the proposed immunosensor. The real effectiveness and feasibility of an LPG-based biosensor were demonstrated by using a complex matrix consisting of human serum, which also confirmed the specificity of the assay, and a limit of detection of 70 µg L(-1) (460 pM) was achieved. |
| ISSN | 09565663 |
| Volume Number | 60 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-10-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Instrumentation Fiber Optic Technology Immunoassay Immunoglobulin G Analysis Immunology Refractometry Equipment Design Equipment Failure Analysis Reproducibility Of Results Sensitivity And Specificity Staining And Labeling Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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