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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Dai, Shuang Feng, Chunjing Li, Wei Wang, Lei Jiang, Wei |
| Description | Author Affiliation: Dai S ( Key Laboratory of Natural Products Chemical Biology, Ministry of Education, School of Pharmaceutical Sciences, Shandong University, 250012 Jinan, PR China.); Feng C ( Key Laboratory of Natural Products Chemical Biology, Ministry of Education, School of Pharmaceutical Sciences, Shandong University, 250012 Jinan, PR China.); Li W ( Key Laboratory of Natural Products Chemical Biology, Ministry of Education, School of Pharmaceutical Sciences, Shandong University, 250012 Jinan, PR China.); Jiang W ( School of Chemistry and Chemical Engineering, Shandong University, 250100 Jinan, PR China. Electronic address: wjiang@sdu.edu.cn.); Wang L ( Key Laboratory of Natural Products Chemical Biology, Ministry of Education, School of Pharmaceutical Sciences, Shandong University, 250012 Jinan, PR China. Electronic address: wangl-sdu@sdu.edu.cn.) |
| Abstract | This work reports a novel and sensitive quantitative method for detection of tumor necrosis factor- (TNF- ) based on single molecule counting and hybridization chain reaction (HCR). In the presence of TNF- , sandwich-type immunocomplex was formed on the surface of glass substrate. The streptavidin acted as a bridge bounded to the biotinylated immunocomplex, which provided three sites to fixate the biotinylated initiator strands. The initiator strands triggered the chain reaction of hybridization to form a long double-helix polymer and SYBR Green I, acted as the fluorescence label, intercalated into the grooves of the long dsDNA polymer. Then, the quantitative detection of TNF- was realized by single molecule counting. Under the optimal conditions, HCR-based single molecule counting quantitative method could successfully detect TNF- in the range of 50 fM to 1 pM, and it revealed a reliable result for TNF- detection in real serum. Moreover, the proposed immunosensor exhibited excellent specificity. These results greatly demonstrated that the proposed method possessed the potentiality in clinical application and it was suitable for quantification of biomarker under low concentration. |
| ISSN | 09565663 |
| Volume Number | 60 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-10-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Dna Genetics Immunoassay Instrumentation In Situ Hybridization, Fluorescence Microchemistry Microscopy, Fluorescence Molecular Imaging Tumor Necrosis Factor-alpha Blood Biosensing Techniques Equipment Design Equipment Failure Analysis Nucleic Acid Amplification Techniques Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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