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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Cali, K. Tremillon, N. Manai, R. Ghassemi, F. Bergonzo, P. Scorsone, E. Possas Abreu, M. Rousseau, L. Lissorgues, G. Arnault, J-C Bernabei, M. Persaud, K. C. Ginisty, H. Tuccori, E. |
| Description | Country affiliation: France Author Affiliation: Manai R ( CEA, LIST, Diamond Sensor Laboratory, 91191, Gif-sur-Yvette, France.); Scorsone E ( ESIEE-Paris, ESYCOM University Paris-EST, Cité Descartes BP99, 93162 Noisy le Grand Cedex, France. Electronic address: emmanuel.scorsone@cea.fr.); Rousseau L ( ESIEE-Paris, ESYCOM University Paris-EST, Cité Descartes BP99, 93162 Noisy le Grand Cedex, France.); Ghassemi F ( ESIEE-Paris, ESYCOM University Paris-EST, Cité Descartes BP99, 93162 Noisy le Grand Cedex, France.); Possas Abreu M ( ESIEE-Paris, ESYCOM University Paris-EST, Cité Descartes BP99, 93162 Noisy le Grand Cedex, France.); Lissorgues G ( ESIEE-Paris, ESYCOM University Paris-EST, Cité Descartes BP99, 93162 Noisy le Grand Cedex, France.); Tremillon N ( The University of Manchester, School of Chemical Engineering and Analytical Science, Oxford Road, Manchester, M13 9PL, UK.); Ginisty H ( The University of Manchester, School of Chemical Engineering and Analytical Science, Oxford Road, Manchester, M13 9PL, UK.); Arnault JC ( CEA, LIST, Diamond Sensor Laboratory, 91191, Gif-sur-Yvette, France.); Tuccori E ( The University of Manchester, School of Chemical Engineering and Analytical Science, Oxford Road, Manchester, M13 9PL, UK.); Bernabei M ( GTP Technology, Immeuble Biostep, 436 Rue Pierre et Marie Curie, 31670 Labège, France.); Cali K ( GTP Technology, Immeuble Biostep, 436 Rue Pierre et Marie Curie, 31670 Labège, France.); Persaud KC ( GTP Technology, Immeuble Biostep, 436 Rue Pierre et Marie Curie, 31670 Labège, France.); Bergonzo P ( CEA, LIST, Diamond Sensor Laboratory, 91191, Gif-sur-Yvette, France.) |
| Abstract | Odorant binding proteins (OBPs) are small soluble proteins found in olfactory systems that are capable of binding several types of odorant molecules. Cantilevers based on polycrystalline diamond surfaces are very promising as chemical transducers. Here two methods were investigated for chemically grafting porcine OBPs on polycrystalline diamond surfaces for biosensor development. The first approach resulted in random orientation of the immobilized proteins over the surface. The second approach based on complexing a histidine-tag located on the protein with nickel allowed control of the proteins' orientation. Evidence confirming protein grafting was obtained using electrochemical impedance spectroscopy, fluorescence imaging and X-ray photoelectron spectroscopy. The chemical sensing performances of these OBP modified transducers were assessed. The second grafting method led to typically 20% more sensitive sensors, as a result of better access of ligands to the proteins active sites and also perhaps a better yield of protein immobilization. This new grafting method appears to be highly promising for further investigation of the ligand binding properties of OBPs in general and for the development of arrays of non-specific biosensors for artificial olfaction applications. |
| ISSN | 09565663 |
| Volume Number | 60 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-10-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biomimetic Materials Diamond Chemistry Dielectric Spectroscopy Instrumentation Micro-electrical-mechanical Systems Odors Analysis Receptors, Odorant Smell Equipment Design Equipment Failure Analysis Protein Binding Protein Interaction Mapping Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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