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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Tahirbegi, Islam Bogachan Mayr, Torsten Sulzer, Philipp Zieger, Silvia Strobl, Martin Kasjanow, Alice Bouwes, Dominique Ehgartner, Josef Paradiso, Mirco |
| Description | Author Affiliation: Tahirbegi IB ( Micronit GmbH, Konrad-Adenauer-Allee 11, Dortmund, 44263 Germany. Electronic address: Bogachan.Tahirbegi@micronit.de.); Ehgartner J ( Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Graz, Austria.); Sulzer P ( Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Graz, Austria.); Zieger S ( Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Graz, Austria.); Kasjanow A ( Micronit GmbH, Konrad-Adenauer-Allee 11, Dortmund, 44263 Germany.); Paradiso M ( Micronit GmbH, Konrad-Adenauer-Allee 11, Dortmund, 44263 Germany.); Strobl M ( Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Graz, Austria.); Bouwes D ( Micronit GmbH, Konrad-Adenauer-Allee 11, Dortmund, 44263 Germany.); Mayr T ( Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Graz, Austria.) |
| Abstract | The necessities of developing fast, portable, cheap and easy to handle pesticide detection platforms are getting attention of scientific and industrial communities. Although there are some approaches to develop microchip based pesticide detection platforms, there is no compact microfluidic device for the complementary, fast, cheap, reusable and reliable analysis of different pesticides. In this work, a microfluidic device is developed for in-situ analysis of pesticide concentration detected via metabolism/photosynthesis of Chlamydomonas reinhardtii algal cells (algae) in tap water. Algae are grown in glass based microfluidic chip, which contains integrated optical pH and oxygen sensors in a portable system for on-site detection. In addition, intrinsic algal fluorescence is detected to analyze the pesticide concentration in parallel to pH and oxygen sensors with integrated fluorescence detectors. The response of the algae under the effect of different concentrations of pesticides is evaluated and complementary inhibition effects depending on the pesticide concentration are demonstrated. The three different sensors allow the determination of various pesticide concentrations in the nanomolar concentration range. The miniaturized system provides the fast quantification of pesticides in less than 10min and enables the study of toxic effects of different pesticides on Chlamydomonas reinhardtii green algae. Consequently, the microfluidic device described here provides fast and complementary detection of different pesticides with algae in a novel glass based microfluidic device with integrated optical pH, oxygen sensors and algal fluorescence. |
| ISSN | 09565663 |
| Volume Number | 88 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2017-02-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Instrumentation Chlamydomonas Reinhardtii Drug Effects Microfluidic Analytical Techniques Oxygen Analysis Pesticides Water Pollutants, Chemical Atrazine Toxicity Physiology Chlorophyta Diuron Drinking Water Equipment Design Fluorescence Hydrogen-ion Concentration Lab-on-a-chip Devices Metabolism Photosynthesis Simazine Journal Article Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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