NDLI: Microfluidic Device for Coulometric Detection of Organophosphate Pesticides.

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Microfluidic Device for Coulometric Detection of Organophosphate Pesticides.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wang, Jin Satake, Takaaki Suzuki, Hiroaki
Description	Author Affiliation: Wang J ( Graduate School of Life and Environmental Sciences, University of Tsukuba.)
Abstract	A microdevice for coulometric detection of organophosphate pesticides (OPs) was fabricated based on the measurement of the inhibition of an enzyme, acetylcholinesterase (AChE), by OPs. Thiocholine (TCh) produced in the enzymatic reaction of AChE with acetylthiocholine (ATCh) as a substrate was oxidized on a microelectrode array formed in a main flow channel. Volumes of plugs of necessary solutions were measured using a structure consisting of a row of rhombuses formed in an auxiliary flow channel. The plugs were merged and solution components were mixed at a T-junction formed with the main and auxiliary flow channels. A linear relationship was confirmed between the generated charge and the logarithm of the OP (malathion) concentration in a concentration range between 10(-6) and 10(-3) M with a correlation coefficient of 0.951. The lower limit of detection was 412 nM.
File Format	HTM / HTML
ISSN	09106340
e-ISSN	13482246
Journal	Analytical Sciences
Issue Number	7
Volume Number	31
Language	English
Publisher	The Japan Society for Analytical Chemistry
Publisher Date	2015-01-01
Publisher Place	Japan
Access Restriction	Open
Subject Keyword	Discipline Analytical Discipline Chemistry Techniques Biosensing Techniques Instrumentation Cholinesterase Inhibitors Lab-on-a-chip Devices Malathion Acetylcholinesterase Metabolism Acetylthiocholine Animals Chemistry Pharmacology Electrophorus Microelectrodes Oxidation-reduction Thiocholine
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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