NDLI: Comparison of optomagnetic and AC susceptibility readouts in a magnetic nanoparticle agglutination assay for detection of C-reactive protein.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Fock, Jeppe Strömberg, Mattias Donolato, Marco Hansen, Mikkel Fougt Svedlindh, Peter Parmvi, Mattias
Description	Author Affiliation: Fock J ( Department of Micro- and Nanotechnology, DTU Nanotech, Technical University of Denmark, Building 345C, Kgs. Lyngby, DK-2800 Denmark. Electronic address: JepF@nanotech.dtu.dk.); Parmvi M ( Department of Micro- and Nanotechnology, DTU Nanotech, Technical University of Denmark, Building 345C, Kgs. Lyngby, DK-2800 Denmark. Electronic address: Mattias.parmvi@blusense-diagnostics.com.); Strömberg M ( Department of Engineering Sciences, Uppsala University, Box 534, Uppsala, SE-751 21 Sweden. Electronic address: Mattias.Stromberg@angstrom.uu.se.); Svedlindh P ( Department of Engineering Sciences, Uppsala University, Box 534, Uppsala, SE-751 21 Sweden. Electronic address: Peter.Svedlindh@angstrom.uu.se.); Donolato M ( Department of Micro- and Nanotechnology, DTU Nanotech, Technical University of Denmark, Building 345C, Kgs. Lyngby, DK-2800 Denmark. Electronic address: Marco@blusense-diagnostics.com.); Hansen MF ( Department of Micro- and Nanotechnology, DTU Nanotech, Technical University of Denmark, Building 345C, Kgs. Lyngby, DK-2800 Denmark. Electronic address: Mikkel.Hansen@nanotech.dtu.dk.)
Abstract	There is an increasing need to develop biosensor methods that are highly sensitive and that can be combined with low-cost consumables. The use of magnetic nanoparticles (MNPs) is attractive because their detection is compatible with low-cost disposables and because application of a magnetic field can be used to accelerate assay kinetics. We present the first study and comparison of the performance of magnetic susceptibility measurements and a newly proposed optomagnetic method. For the comparison we use the C-reactive protein (CRP) induced agglutination of identical samples of 100nm MNPs conjugated with CRP antibodies. Both methods detect agglutination as a shift to lower frequencies in measurements of the dynamics in response to an applied oscillating magnetic field. The magnetic susceptibility method probes the magnetic response whereas the optomagnetic technique probes the modulation of laser light transmitted through the sample. The two techniques provided highly correlated results upon agglutination when they measure the decrease of the signal from the individual MNPs (turn-off detection strategy), whereas the techniques provided different results, strongly depending on the read-out frequency, when detecting the signal due to MNP agglomerates (turn-on detection strategy). These observations are considered to be caused by differences in the volume-dependence of the magnetic and optical signals from agglomerates. The highest signal from agglomerates was found in the optomagnetic signal at low frequencies.
ISSN	09565663
Volume Number	88
e-ISSN	18734235
Journal	Biosensors and Bioelectronics
Language	English
Publisher	Elsevier
Publisher Date	2017-02-15
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Antibodies, Immobilized Chemistry Biosensing Techniques Methods C-reactive Protein Analysis Magnetite Nanoparticles Agglutination Tests Animals Goats Humans Limit Of Detection Magnetics Comparative Study Journal Article Discipline Biotechnology
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry

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