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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bernot, Kelsie M. Lee, Chang-hun Coulombe, Pierre A. |
| Description | Author Affiliation: Bernot KM ( Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.) |
| Abstract | Intermediate filaments (IFs) are fibrous polymers encoded by a large family of differentially expressed genes that provide crucial structural support in the cytoplasm and nucleus in higher eukaryotes. The mechanisms involved in bringing together ∼16 elongated coiled-coil dimers to form an IF are poorly defined. Available evidence suggests that tetramer subunits play a key role during IF assembly and regulation. Through molecular modeling and site-directed mutagenesis, we document a hitherto unnoticed hydrophobic stripe exposed at the surface of coiled-coil keratin heterodimers that contributes to the extraordinary stability of heterotetramers. The inability of K16 to form urea-stable tetramers in vitro correlates with an increase in its turnover rate in vivo. The data presented support a specific conformation for the assembly competent IF tetramer, provide a molecular basis for their differential stability in vitro, and point to the physiological relevance associated with this property in vivo. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 6 |
| Volume Number | 168 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 2005-03-14 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Hydrophobic And Hydrophilic Interactions Keratins Chemistry Metabolism Amino Acid Sequence Amino Acid Substitution Animals Cells, Cultured Chromatography, Ion Exchange Consensus Sequence Cross-Linking Reagents Pharmacology Dimerization Electrophoresis, Agar Gel Electrophoresis, Polyacrylamide Gel Escherichia Coli Genetics Keratinocytes Ultrastructure Mice Models, Molecular Molecular Sequence Data Mutagenesis, Site-Directed Precipitin Tests Protein Conformation Protein Structure, Tertiary Recombinant Proteins Isolation & Purification Sequence Homology, Amino Acid Comparative Study Research Support, U.S. Gov't, P.H.S. Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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