NDLI: Kinetics of cell death and disintegration in human lymphocyte cultures.

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Kinetics of cell death and disintegration in human lymphocyte cultures.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bernheim, J. L. Kelley, M. F. Dorian, R. Mendelsohn, J.
Abstract	In order to quantitate lymphocyte proliferative responses, we explored the role of cell death in the kinetics of phytohemagglutinin-stimulated cultures. Unless the disintegration time (tDIS) of nonviable lymphocytes in culture is known, the rate of cell death cannot be calculated. To obtain tDIS, we determined the time interval between total and viable cell population decay after various killing events. Two subpopulations of lymphocytes were observed, the major (80%) with a mean (+/-SEM) tDIS of 16+/-2 hr and the minor (20%) with a tDIS of 45+/-7 hr. Kinetic balance sheets were constructed predicting total culture DNA content (cells plus medium), as calculated both from proliferation rates and from observed death and disintegration rates. In an experiment characterized by extensive cell death, the two tallies were well-matched when the above data were utilized. The large discrepancy between predicted and observed DNA contents of the medium indicates that the DNA of disintegrated lymphocytes is extensively degraded. We conclude that cell death explains proliferation deficits in stimulated lymphocyte cultures. Our approach to quantitation of cell death may have general applicability to kinetic studies of cultured cells.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	6
Volume Number	74
Language	English
Publisher	National Academy of Sciences
Publisher Date	1977-09-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Lymphocytes Physiology Cell Division Cell Survival Drug Effects Radiation Effects Cells, Cultured Cyanides Pharmacology Cytarabine Demecolcine Kinetics Lectins Lymphocyte Activation Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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