NDLI: Ligand-promoted weakening of intersubunit bonding domains in aspartate transcarbamolylase.

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Ligand-promoted weakening of intersubunit bonding domains in aspartate transcarbamolylase.

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Ligand-promoted weakening of intersubunit bonding domains in aspartate transcarbamolylase.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Schachman, H. K. Subramani, S. Bothwell, M. A. Yang, Y. R. Gibbons, I.
Abstract	THE COOPERATIVITY AND FEEDBACK INHIBITION EXHIBITED BY THE REGULATORY ENZYME, ASPARTATE TRANSCARBAMOYLASE (CARBAMOYLPHOSPHATE: L-aspartate carbamoyltransferase; EC 2.1.3.2), from Escherichia coli are generally attributed to ligand-promoted conformational changes involving alterations in the subunit interactions. However, no quantitative estimates have been made of the effect of ligands on the strength of the intersubunit bonding domains. The native enzyme, composed of two catalytic trimers 'crosslinked' by three regulatory dimers, shows little tendency to dissociate in neutral buffers at room temperature. In addition, very little exchange was observed in 2 hr between subunits within the intact enzyme and free subunits. Although exchange was enhanced in solutions of low ionic strength containing the bisubstrate analog, N-(phosphonacetyl)-L-aspartate, the rates of exchange were too small to permit reliable estimates of the weakening of the bonding domains caused by the ligand. Studies were conducted, therefore, on a less stable oligomeric complex which resembles the native enzyme in structure and allosteric behavior but lacks one regulatory subunit. These molecules, containing only four bonding domains between the catalytic and regulatory polypeptide chains (compared to six in the native enzyme), disproportionate to form the more stable native enzyme and free catalytic subunits. An electrophoretic technique is described for measuring the rate of disproportionation which is controlled by the rupture of the intersubunit bonding domains. This rate is enhanced about 300-fold upon the addition of the active-site ligand. Hence the ligand-promoted allosteric conversion of the enzyme-like complex from the constrained to the relaxed conformation involves a substantial weakening of the intersubunit interactions corresponding to about 1.7 kcal/mole (7.1 kJ/mole) per bonding domain between a catalytic and a regulatory chain.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	9
Volume Number	74
Language	English
Publisher	National Academy of Sciences
Publisher Date	1977-11-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Aspartate Carbamoyltransferase Metabolism Aspartic Acid Binding Sites Drug Stability Escherichia Coli Enzymology Kinetics Ligands Macromolecular Substances Organophosphonates Osmolar Concentration Temperature Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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