NDLI: High aerobic glycolysis of rat hepatoma cells in culture: role of mitochondrial hexokinase.

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High aerobic glycolysis of rat hepatoma cells in culture: role of mitochondrial hexokinase.

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High aerobic glycolysis of rat hepatoma cells in culture: role of mitochondrial hexokinase.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bustamante, E. Pedersen, P. L.
Abstract	A tumorigenic anchorage-dependent cell line (H-91) was established in culture from an azo-dye-induced rat ascites hepatoma. When grown in a glucose-containing medium the cells exhibit high rates of lactic acid production characteristic of rapidly growing tumor cells. However, when glucose is replaced with galactose the cells grow equally well but exhibit only moderately elevated rates of lactic acid production. The molecular basis for this observation cannot be attributed to differences in permeability because initial rates of glucose and galactose entry into hepatoma cells are identical. Rather, the activity of hexokinase (ATP:D-hexose 6-phosphotransferase, EC 2.7.1.1) is found to be high in hepatoma cells, about 20-fold higher than that of control and regenerating rat liver. Moreover, tumor hexokinase activity is not inhibited by low concentrations (<0.6 mM) of the reaction product glucose 6-phosphate. Additionally, 50% of the hexokinase activity of hepatoma cells is found associated with the mitochondrial fraction. This fraction is 3-fold enriched in hexokinase activity relative to the homogenate and 4-fold enriched relative to the nuclear and postmitochondrial fractions. Tumor mitochondrial hexokinase appears to be coupled directly to oxidative phosphorylation, because addition of glucose to respiring hepatoma mitochondria (after a burst of ATP synthesis) results in stimulation of respiration. In contrast, glucose has no effect on the respiration of mitochondria from control and regenerating liver. These results suggest that the high glycolytic capacity of H-91 hepatoma cells is due, at least in part, to an elevated form of hexokinase concentrated in the mitochondrial fraction of the cell.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	9
Volume Number	74
Language	English
Publisher	National Academy of Sciences
Publisher Date	1977-11-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Carcinoma, Hepatocellular Metabolism Glycolysis Hexokinase Mitochondria Enzymology Aerobiosis Animals Biological Transport, Active Cells, Cultured Galactose Glucose Kinetics Liver Neoplasms Neoplasms, Experimental Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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