NDLI: Animal cell mutants defective in sterol metabolism: a specific selection procedure and partial characterization of defects.

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Reconstitution of catecholamine-sensitive adenylate cyclase activity: interactions of solubilized components with receptor-replete membranes.

Single-stranded DNA from oncornavirus-infected cells enriched in virus-specific DNA sequences.

High aerobic glycolysis of rat hepatoma cells in culture: role of mitochondrial hexokinase.

Purification of simian virus 40 tumor antigen from a line of simian virus 40-transformed human cells.

Enzyme regulation in neuroblastoma cells in a salts/glucose medium: induction of ornithine decarboxylase by asparagine and glutamine.

Interaction of L-alpha-didecanoyl phosphatidylcholine with the AI polypeptide of high density lipoprotein.

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Division of BALB/c mouse 3T3 and simian virus 40-transformed 3T3 cells in cellular aggregates.

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Amyloid protein SAA is associated with high density lipoprotein from human serum.

Specific receptors for des-Asp1-angiotensin II (('angiotensin III') in rat adrenals.

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Animal cell mutants defective in sterol metabolism: a specific selection procedure and partial characterization of defects.

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Loss of growth control and differentiation in the fu-1 variant of the L8 line of rat myoblasts.

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Rapid speciation and chromosomal evolution in mammals.

Overexpression of an unstably inherited gene in cultured mouse cells.

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Antibodies to tubulin in normal nonimmunized animals.

Inhibition of human platelet thromboxane synthetase by 9,11-azoprosta-5,13-dienoic acid.

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Neurophysin biosynthesis in normal rats and in rats with hereditary diabetes insipidus.

Effectof MgATP on stiffness measured at two frequencies in Ca2+-activated muscle fibers.

Variants of 3T3 cells lacking mitogenic response to epidermal growth factor.

beta-Endorphin: development of tolerance and its reversal by 5-hydroxytryptophan in cats.

Transport of alpha-amylase across the basolateral membrane of the pancreatic acinar cell.

Mutator action by Escherichia coli strains carrying dnaE mutations.

Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.

Electron microscope studies of transient complexes formed between Escherichia coli RNA polymerase holoenzyme and T7 DNA.

Ligand-promoted weakening of intersubunit bonding domains in aspartate transcarbamolylase.

Cell cycle of Saccharomycescerevisiae in populations growing at different rates.

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Terminus region of the chromosome in Escherichia coli inhibits replication forks.

Involvement of DNA-dependent RNA polymerase in a recA-independent pathway of genetic recombination in Escheria coli.

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Animal cell mutants defective in sterol metabolism: a specific selection procedure and partial characterization of defects.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Saito, Y. Chou, S. M. Silbert, D. F.
Abstract	By using a chemically defined medium, a general and highly specific procedure was devised to select for mutant cells with less abundant or structurally altered sterol in their surface membranes. Within a certain concentration range, the polyene antibiotic filipin was shown to kill only cells with normal (as opposed to decreased) membrane sterol levels. Sterol-requiring derivatives of LM cells were isolated by chemical mutagenesis, filipin treatment, and cloning followed by replica plating in soft agar. Mutants (S1 and S2) are described which, when compared to normal cells, show decreased synthesis of demosterol in vivo from acetate and mevalonate relative to cell number or to fatty acid synthesis. When exogenous sterol is supplied, mutants S1 and S2 grow normally in suspension culture. However, when deprived of sterol supplement, mutant S1 grows slower than wild type cells and mutant S2 lyses within one to two generations. Gas/liquid chromatography revealed that the mutants contained a normal spectrum of fatty acids including unsaturated fatty acyl groups but, unlike wildtype cells, they have less abundant (mutant S1) or no (mutant S2) desmosterol in either the presence or absence of exogenous cholesterol. In vitro experiments with mevalonate as the substrate suggest that the defect in both mutants is in a demethylation reaction subsequent to lanosterol synthesis. The selection method developed here may permit the isolation of mutants with defective membrane incorporation of sterols and other polyisoprenoids as well as defective synthesis of these compounds.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	9
Volume Number	74
Language	English
Publisher	National Academy of Sciences
Publisher Date	1977-11-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Mutation Sterols Genetics Acetates Metabolism Cell Division Drug Effects Cell Line Cholesterol Desmosterol Filipin Pharmacology Hydroxycholesterols Methylnitronitrosoguanidine Mevalonic Acid Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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