NDLI: Tightly bound nuclear progesterone receptor is not phosphorylated in primary chick oviduct cultures.

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Tightly bound nuclear progesterone receptor is not phosphorylated in primary chick oviduct cultures.

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Tightly bound nuclear progesterone receptor is not phosphorylated in primary chick oviduct cultures.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Jung-testas, I. Garcia, T. Baulieu, E. E.
Abstract	Oviduct cells from estradiol-treated chicks were grown in primary culture. After 3-5 days of culture in medium containing estradiol, 90% of the cellular progesterone binding sites were detected in the cytosol. After exposure to [3H]progesterone at 37 degrees C, 80% of the progesterone binding sites were found in nuclear fractions. Progesterone receptor phosphorylation was assessed after incubating the cells with [32P]orthophosphate. Receptor components were immunoprecipitated with a specific polyclonal antibody (IgG-G3) and analyzed by NaDodSO4/PAGE and autoradiography. In the cytosol, constant amounts of 32P-labeled 110-kDa subunit (the B subunit, one of the progesterone-binding components of the receptor) and of the non-steroid-binding heat shock protein hsp90 were found, whether cells had been exposed to progesterone or not. No 32P-labeled 79-kDa subunit (the A subunit, another progesterone-binding subunit) was detected. Various procedures were used to solubilize nuclear progesterone receptor (0.5 M KCl, micrococcal nuclease, NaDodSO4), and in no case was 32P-labeled B subunit detected in the extracts. However, nonradioactive B subunit was detected by immunoblot in a nuclear KCl extract of progesterone-treated cells. These results suggest that the fraction of the B subunit that becomes strongly attached to nuclear structures is not phosphorylated upon exposure of cells to progesterone.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	20
Volume Number	83
Language	English
Publisher	National Academy of Sciences
Publisher Date	1986-11-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cell Nucleus Metabolism Oviducts Receptors, Progesterone Animals Cells, Cultured Cytosol Immunoglobulin G Immunology Phosphorylation Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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