NDLI: On the spin trapping and ESR detection of oxygen-derived radicals generated inside cells.

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On the spin trapping and ESR detection of oxygen-derived radicals generated inside cells.

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On the spin trapping and ESR detection of oxygen-derived radicals generated inside cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Carmichael, A. J. Samuni, A. Russo, A. Mitchell, J. B. Riesz, P.
Abstract	Recently several attempts to identify oxygen-derived radicals in whole cells by spin trapping and electron spin resonance have been reported by using 5,5-dimethyl-1-pyrroline-N-oxide as the spin trap. In the present study, the feasibility of this method is examined. Chinese hamster V79 cells and human erythrocytes served as the test systems, while OH radicals were generated by gamma radiolysis. Several spin traps were used to scavange the radicals and a distinction between exo- and endocellular ESR observable species was achieved using tri(oxalato) chromiate(III) as a line broadening agent. To distinguish between exo- and endocellular sites of radical formation, we studied the effects of high molecular weight scavengers (polyethylene glycols), which do not enter the cell. Various possible obstacles associated with trapping and detecting the radicals inside the cells were examined. The results indicate that the primary radicals react with the spin traps. However, these spin adducts decayed within the cells. Cellularly induced decay of 2-hydroxy-5,5-dimethyl-1-pyrrolidinyloxyl radical presented the major difficulty in detecting the endogenous radicals, and potential experimental approaches to overcome this difficulty are discussed.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	20
Volume Number	83
Language	English
Publisher	National Academy of Sciences
Publisher Date	1986-11-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Hydroxides Superoxides Animals Cell Line Cricetinae Cyclic N-Oxides Electron Spin Resonance Spectroscopy Free Radicals Hydroxyl Radical Molecular Weight Polyethylene Glycols Pharmacology Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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