NDLI: Heterologous expression of a wheat high molecular weight glutenin gene in Escherichia coli.

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Heterologous expression of a wheat high molecular weight glutenin gene in Escherichia coli.

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Heterologous expression of a wheat high molecular weight glutenin gene in Escherichia coli.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Galili, G.
Description	Author Affiliation: Galili G ( Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel.);
Abstract	An engineered DNA fragment containing a DNA sequence encoding a wheat high molecular weight glutenin subunit was cloned into a bacterial expression vector that is based on bacteriophage T7 RNA polymerase. The resulting plasmid directed the synthesis of large amounts of the mature form of the wheat high molecular weight glutenin subunit in Escherichia coli. This protein comigrated in SDS/PAGE with a native high molecular weight glutenin subunit extracted from wheat endosperm and cross-reacted with antibodies raised against purified wheat high molecular weight glutenins. The wheat subunit synthesized in E. coli also exhibited pI and solubility characteristics identical to those of native wheat subunits. Moreover, the wheat glutenin subunit produced in E. coli cells self-assembled into oligomers linked by intermolecular disulfide bonds, a process that plays an important role in the assembly of native glutenins during gluten formation. The large amounts of a purified wheat subunit obtained from E. coli will enable investigators to analyze the three-dimensional structure of that protein and to identify protein sequences that affect the bread-making quality of wheat dough.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	20
Volume Number	86
Language	English
Publisher	National Academy of Sciences
Publisher Date	1989-12-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Escherichia Coli Genetics Genes, Plant DNA Gene Expression Glutens Isolation & Purification Macromolecular Substances Molecular Weight Recombinant Proteins Triticum Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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