NDLI: Immunoglobulin double-isotype expression by trans-mRNA in a human immunoglobulin transgenic mouse.

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Specific photoaffinity labeling of two plasma membrane polypeptides with an azido auxin.

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Immunoglobulin double-isotype expression by trans-mRNA in a human immunoglobulin transgenic mouse.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Mizuta, T. R. Leder, P. Honjo, T. Shimizu, A. Nussenzweig, M. C.
Description	Author Affiliation: Shimizu A ( Center for Molecular Biology and Genetics, Kyoto University, Japan.);
Abstract	We have studied immunoglobulin double-isotype expression in a transgenic mouse (TG.SA) in which expression of the endogenous immunoglobulin heavy chain locus is almost completely excluded by a nonallelic rearranged human mu transgene. By flow-cytometric analyses, we have shown that a small, but significant, portion (about 4%) of transgenic spleen cells expresses human mu (transgene) and mouse gamma (endogenous) chains when cultured in vitro with bacterial lipopolysaccharide and interleukin 4. By using amplification of cDNA by the polymerase chain reaction, followed by cloning and sequencing of the amplified cDNA fragment, we have demonstrated expression of trans-mRNA consisting of the transgenic variable and endogenous constant (gamma 1) region sequences. Such trans-mRNA could be produced by either switch recombination or trans-splicing between the transgene and endogenous sterile gamma 1-gene transcripts. These results indicate that trans-splicing might be a possible mechanism for the immunoglobulin double-isotype expression in normal B lymphocytes that have not rearranged the second expressed constant region gene.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	20
Volume Number	86
Language	English
Publisher	National Academy of Sciences
Publisher Date	1989-12-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Gene Expression Genes, Immunoglobulin Immunoglobulin Isotypes Genetics Lymphocytes Immunology RNA, Messenger Animals Exons Flow Cytometry Immunoglobulin Heavy Chains Immunoglobulin Mu-Chains Mice Mice, Transgenic Molecular Sequence Data Polymerase Chain Reaction RNA Splicing Spleen Transcription, Genetic Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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