NDLI: Transcription of the testis-specific mouse protamine 2 gene in a homologous in vitro transcription system.

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Transcription of the testis-specific mouse protamine 2 gene in a homologous in vitro transcription system.

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Transcription of the testis-specific mouse protamine 2 gene in a homologous in vitro transcription system.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bunick, D. Johnson, P. A. Hecht, N. B. Johnson, T. R.
Description	Author Affiliation: Bunick D ( Department of Biology, Tufts University, Medford, MA 02155.);
Abstract	Transcriptionally active nuclear extracts were prepared from mouse testes to study the transcription of the testis-specific mouse protamine 2 (Prm-2) gene in vitro. The testicular system is unique among mammalian in vitro transcription systems in regard to its temperature optimum. In extracts made from prepuberal testes, the temperature optimum for in vitro transcription of Prm-2 is 30 degrees C, similar to somatic in vitro systems. However, in adult testis extracts, the optimum temperature for Prm-2 transcription is 20 degrees C. The different temperature optima seen in vitro for prepuberal and adult testes extracts parallels in vivo physiological temperature sensitivities of the differentiating male germ cells. The testis system also differs from other in vitro transcription systems in its divalent metal cation and ionic strength requirements for optimal transcription. The mouse Prm-2 gene is maximally transcribed at a MgCl2 concentration of 3-5 mM and over a KCl concentration range of 40-100 mM. By using the testis in vitro transcription system to study the Prm-2 gene by deletion analysis, we have determined that positive promotion for the gene lies within the region -170 to -82 from the start of transcription. This region contains a putative Sp-1 binding site. Additional upstream sequences appear to repress Prm-2 transcription in a heterologous transcription system.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	3
Volume Number	87
Language	English
Publisher	National Academy of Sciences
Publisher Date	1990-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Genes Protamines Genetics Testis Metabolism Transcription, Genetic Animals Cell Nucleus Chromosome Deletion HeLa Cells Mice Molecular Sequence Data Molecular Weight Restriction Mapping Templates, Genetic Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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