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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Shields, M. T. Amin, M. Mercer, W. E. Appella, E. Ullrich, S. J. Sauve, G. J. Romano, J. W. |
| Description | Author Affiliation: Mercer WE ( Department of Pathology, Temple University School of Medicine, Philadelphia, PA 19140.); |
| Abstract | To investigate the effect that human wild-type p53 (wt-p53) expression has on cell proliferation we constructed a recombinant plasmid, pM47, in which wt-p53 cDNA is under transcriptional control of the hormone-inducible mouse mammary tumor virus promoter linked to the dominant biochemical selection marker gene Eco gpt. The pM47 plasmid was introduced into T98G cells derived from a human glioblastoma multiforme tumor, and a stable clonal cell line, GM47.23, was derived that conditionally expressed wt-p53 following exposure to dexamethasone. We show that induction of wt-p53 expression in exponentially growing cells inhibits cell cycle progression and that the inhibitory effect is reversible upon removal of the inducer or infection with simian virus 40. Moreover, when growth-arrested cells are stimulated to proliferate, induction of wt-p53 expression inhibits G0/G1 progression into S phase and the cells accumulate with a DNA content equivalent to cells arrested in the G0/G1 phase of the cell cycle. Taken together, these studies suggest that wt-p53 may play a negative role in growth regulation. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 16 |
| Volume Number | 87 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1990-09-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Oncogene Proteins Genetics Phosphoproteins Tumor Cells, Cultured Cytology Blotting, Northern Blotting, Southern Cell Cycle Drug Effects Cell Division Cell Line DNA Replication Dexamethasone Pharmacology Glioma Nuclear Proteins RNA, Messenger Isolation & Purification RNA, Neoplasm Thymidine Metabolism Transfection Tumor Suppressor Protein P53 Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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