NDLI: Cellular and peptide requirements for in vitro clonal deletion of immature thymocytes.

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Cellular and peptide requirements for in vitro clonal deletion of immature thymocytes.

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Cellular and peptide requirements for in vitro clonal deletion of immature thymocytes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Iwabuchi, K. Nakayama, K. Murphy, K. M. Loh, D. Y. Nishimura, T. Wang, F. Habu, S. Mccoy, R. L.
Description	Author Affiliation: Iwabuchi K ( Department of Medicine, Genetics, and Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110.);
Abstract	Thymocytes from DO10 T-cell-receptor transgenic mice undergo apoptosis, or programmed cell death, when chicken ovalbumin-(323-339) peptide is administered in vivo. Using DO10 mice thymocytes, we have now developed a simple in vitro model system that recapitulates the in vivo clonal-deletion process. When transgenic thymocytes were cocultured with fibroblasts, B cells, or thymic nurse cell lines (all bearing I-Ad) in the presence of chicken ovalbumin-(323-339), deletion of the transgenic TCR+CD4+CD8+ thymocytes was seen within 8-20 hr. Thymocytes designed to bear I-Ad on their surface could mediate the deletion themselves. Thus, thymocyte clonal deletion entirely depends on the stage at which the thymocytes are vulnerable to the onset of apoptosis, rather than on the nature of the peptide antigen-presenting cells. Furthermore, thymic nurse cell line TNC-R3.1 could cause deletion, strongly suggesting that some thymic epithelial/stromal components are potentially capable of participating in negative selection. In all cases examined, little deletion could be induced at a peptide concentration less than 10 nM, thus defining the minimum amount of peptide antigen required for negative selection. The peptide-dependent in vitro negative-selection system will allow further dissection of the molecular and cellular processes involved in clonal deletion due to apoptosis in the thymus.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	19
Volume Number	89
Language	English
Publisher	National Academy of Sciences
Publisher Date	1992-11-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Lymphocyte Depletion Receptors, Antigen, T-Cell Immunology T-Lymphocytes Amino Acid Sequence Animals Antigen-Presenting Cells Antigens, CD4 Antigens, CD8 Apoptosis B-Lymphocytes Cell Line Cells, Cultured Crosses, Genetic DNA Genetics Isolation & Purification Flow Cytometry Genes, MHC Class II Mice Mice, Inbred BALB C Mice, Transgenic Molecular Sequence Data Ovalbumin Peptides Chemical Synthesis Pharmacology Cytology Drug Effects Thymus Gland Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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