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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ray, D. S. Brown, G. W. Melendy, T. E. |
| Description | Author Affiliation: Brown GW ( Molecular Biology Institute, University of California, Los Angeles 90024.); |
| Abstract | Human replication protein A (RP-A) is a three-subunit protein that is required for simian virus 40 (SV40) replication in vitro. The trypanosome homologue of RP-A has been purified from Crithidia fasciculata. It is a 1:1:1 complex of three polypeptides of 51, 28, and 14 kDa, binds single-stranded DNA via the large subunit, and is localized within the nucleus. C. fasciculata RP-A substitutes for human RP-A in the large tumor antigen-dependent unwinding of the SV40 origin of replication and stimulates both DNA synthesis and DNA priming by human DNA polymerase alpha/primase, but it does not support efficient SV40 DNA replication in vitro. This extraordinary conservation of structure and function between human and trypanosome RP-A suggests that the mechanism of DNA replication, at both the initiation and the elongation level, is conserved in organisms that diverged from the main eukaryotic lineage very early in evolution. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 21 |
| Volume Number | 89 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1992-12-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Crithidia Fasciculata Genetics DNA Replication DNA-Binding Proteins Metabolism Animals Chromatography, Affinity DNA Polymerase II Isolation & Purification DNA-Directed DNA Polymerase Macromolecular Substances Replication Protein A Simian Virus 40 Templates, Genetic Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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