NDLI: Purification of human leukotriene C4 synthase.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1992 Volume: 89 Issue: 24

Year: 1992 Volume: 89 Issue: 23

Casimir energy for dielectrics: spherical geometry.

Reproductive biology of the Permian Glossopteridales and their suggested relationship to flowering plants.

Cloned, expressed rat cerebellar nitric oxide synthase contains stoichiometric amounts of heme, which binds carbon monoxide.

Identification of a pathogenic epitope involved in initiation of Heymann nephritis.

itk, a T-cell-specific tyrosine kinase gene inducible by interleukin 2.

Distinct presynaptic control of dopamine release in striosomal- and matrix-enriched areas of the rat striatum by selective agonists of NK1, NK2, and NK3 tachykinin receptors.

Identification of a human src homology 2-containing protein-tyrosine-phosphatase: a putative homolog of Drosophila corkscrew.

Tyrosine phosphorylation is required for activation of an alpha interferon-stimulated transcription factor.

Selective endothelial binding of interleukin-2-dependent human T-cell lines derived from different tissues.

Transmembrane signaling by the high-affinity IgE receptor on membrane preparations.

DNA binding and heteromerization of the Drosophila transcription factor chorion factor 1/ultraspiracle.

Distinct monoclonal antibodies separately label the hexons or the pentons of herpes simplex virus capsid.

Nitric oxide mediates norepinephrine-induced prostaglandin E2 release from the hypothalamus.

A functional domain in the heavy chain of scatter factor/hepatocyte growth factor binds the c-Met receptor and induces cell dissociation but not mitogenesis.

Genetic control of murine hematopoietic stem cell pool sizes and cycling kinetics.

Marked increase in nitric oxide synthase mRNA in rat dorsal root ganglia after peripheral axotomy: in situ hybridization and functional studies.

Antiserum raised against a synthetic phosphotyrosine-containing peptide selectively recognizes p185neu/erbB-2 and the epidermal growth factor receptor.

High density of Ca(2+)-dependent K+ and Cl- channels on the luminal membrane of lacrimal acinar cells.

Regulation of an opioid-binding protein in NG108-15 cells parallels regulation of delta-opioid receptors.

T-cell receptor beta-chain gene usage in the T-cell recognition of Mycobacterium leprae antigens in one tuberculoid leprosy patient.

Evidence for a base-pairing interaction between U6 small nuclear RNA and 5' splice site during the splicing reaction in yeast.

Increased levels of trkB mRNA and trkB protein-like immunoreactivity in the injured rat and cat spinal cord.

Carbohydrate cycling in signal transduction: parafusin, a phosphoglycoprotein and possible Ca(2+)-dependent transducer molecule in exocytosis in Paramecium.

Quantum aspects of brain activity and the role of consciousness.

The role of endogenous atrial natriuretic peptide in resting and stress-induced release of corticotropin, prolactin, growth hormone, and thyroid-stimulating hormone.

RAD25 (SSL2), the yeast homolog of the human xeroderma pigmentosum group B DNA repair gene, is essential for viability.

Positive supercoiling of DNA greatly diminishes mRNA synthesis in yeast.

Modulation of a transient K+ current in the pleural sensory neurons of Aplysia by serotonin and cAMP: implications for spike broadening.

cAMP modulates multiple K+ currents, increasing spike duration and excitability in Aplysia sensory neurons.

Leukotriene C4 uses a probenecid-sensitive export carrier that does not recognize leukotriene B4.

Molecular cloning and functional expression of a brain-specific somatostatin receptor.

Isolation of transglutaminase-reactive sequences from complex biological systems: a prominent lysine donor sequence in bovine lens.

Enhancement of human immunodeficiency virus (HIV)-specific CD4+ and CD8+ cytotoxic T-lymphocyte activities in HIV-infected asymptomatic patients given recombinant gp160 vaccine.

Toward a cytogenetically based physical map of the wheat genome.

Thymic depletion and peripheral activation of class I major histocompatibility complex-restricted T cells by soluble peptide in T-cell receptor transgenic mice.

Up-regulation of intercellular adhesion molecule 1 transcription by hepatitis B virus X protein.

Tat-responsive region RNA of human immunodeficiency virus type 1 stimulates protein synthesis in vivo and in vitro: relationship between structure and function.

Identification and genetic mapping of a homeobox gene to the 4p16.1 region of human chromosome 4.

Genomic polymorphism, recombination, and linkage disequilibrium in human major histocompatibility complex-encoded antigen-processing genes.

CD8 T cells can protect against an intracellular bacterium in an interferon gamma-independent fashion.

Sp1 and the subfamily of zinc finger proteins with guanine-rich binding sites.

Gene therapy. (Journal: Proceedings of the National Academy of Sciences of the United States of America Volume: 89 Issue: 23)

Implicit knowledge: new perspectives on unconscious processes.

Application of DNA fingerprinting to the recovery program of the endangered Puerto Rican parrot.

Requirement for coenzyme Q in plasma membrane electron transport.

A putative ATP-dependent RNA helicase involved in Saccharomyces cerevisiae ribosome assembly.

Active complete in vitro replication of nodavirus RNA requires glycerophospholipid.

Evidence that the packaging signal for nodaviral RNA2 is a bulged stem-loop.

At least three distinct proteins are necessary for the reconstitution of a specific multiprotein complex at a eukaryotic chromosomal origin of replication.

Marked replicative advantage of human mtDNA carrying a point mutation that causes the MELAS encephalomyopathy.

A yeast cyclophilin gene essential for lactate metabolism at high temperature.

Isolation, characterization, and transcription of the gene encoding mouse mast cell protease 7.

Expression of a coriander desaturase results in petroselinic acid production in transgenic tobacco.

Fibrinogen blocks the autoactivation and thrombin-mediated activation of factor XI on dextran sulfate.

Relationship between O6-alkylguanine-DNA alkyltransferase activity and N-methyl-N'-nitro-N-nitrosoguanidine-induced mutation, transformation, and cytotoxicity in C3H/10T1/2 cells expressing exogenous alkyltransferase genes.

Specific inhibition of human immunodeficiency virus type 1 replication by antisense oligonucleotides: an in vitro model for treatment.

Transcription of the hypersensitive site HS2 enhancer in erythroid cells.

General splicing factors SF2 and SC35 have equivalent activities in vitro, and both affect alternative 5' and 3' splice site selection.

Multiple growth factors, cytokines, and neurotrophins rescue photoreceptors from the damaging effects of constant light.

Binding and transport of gangliosides by prosaposin.

Basal release of nitric oxide from aortic rings is greater in female rabbits than in male rabbits: implications for atherosclerosis.

Control of gal transcription through DNA looping: inhibition of the initial transcribing complex.

NAD+ and NADH regulate an ATP-dependent kinase that phosphorylates enzyme I of the Escherichia coli phosphotransferase system.

Aerosol gene delivery in vivo.

Molecular cloning of the gene for the yeast homolog (ACB) of diazepam binding inhibitor/endozepine/acyl-CoA-binding protein.

Yeast RNA polymerase II initiation factor e: isolation and identification as the functional counterpart of human transcription factor IIB.

Drosophila homolog of the human S6 ribosomal protein is required for tumor suppression in the hematopoietic system.

Probucol inhibits neointimal thickening and macrophage accumulation after balloon injury in the cholesterol-fed rabbit.

Structural analysis of the human interferon gamma receptor: a small segment of the intracellular domain is specifically required for class I major histocompatibility complex antigen induction and antiviral activity.

Drug design by machine learning: the use of inductive logic programming to model the structure-activity relationships of trimethoprim analogues binding to dihydrofolate reductase.

Stable heparin-producing cell lines derived from the Furth murine mastocytoma.

Efficient transfer and sustained high expression of the human glucocerebrosidase gene in mice and their functional macrophages following transplantation of bone marrow transduced by a retroviral vector.

Human fusion proteins between interleukin 2 and IgM heavy chain are cytotoxic for cells expressing the interleukin 2 receptor.

Brain-derived neurotrophic factor augments rotational behavior and nigrostriatal dopamine turnover in vivo.

Molecular characterization of NAD:arginine ADP-ribosyltransferase from rabbit skeletal muscle.

Effect of Ca2+ on weak cross-bridge interaction with actin in the presence of adenosine 5'-[gamma-thio]triphosphate).

pH gradients across phospholipid membranes caused by fast flip-flop of un-ionized fatty acids.

Vibronic energy map and excited state vibrational characteristics of magnesium myoglobin determined by energy-selective fluorescence.

Differential effects of Bcl-2 on T and B cells in transgenic mice.

Major histocompatibility complex class I-specific and -restricted killing of beta 2-microglobulin-deficient cells by CD8+ cytotoxic T lymphocytes.

Introduction of a retinoid reporter gene into the urodele limb blastema.

Epidermal growth factor stimulates mouse placental lactogen I but inhibits mouse placental lactogen II secretion in vitro.

Phage phi 29 regulatory protein p4 stabilizes the binding of the RNA polymerase to the late promoter in a process involving direct protein-protein contacts.

Intramolecular DNA triplexes: unusual sequence requirements and influence on DNA polymerization.

Malignancy of eye melanomas originating in the retinal pigment epithelium of transgenic mice after genetic ablation of choroidal melanocytes.

A proposed function for spermine and spermidine: protection of replicating DNA against damage by singlet oxygen.

Spermine and spermidine protection of plasmid DNA against single-strand breaks induced by singlet oxygen.

Reading-frame restoration with an apolipoprotein B gene frameshift mutation.

Activin inhibits binding of transcription factor Pit-1 to the growth hormone promoter.

Additional antitumor ecteinascidins from a Caribbean tunicate: crystal structures and activities in vivo.

Methodologic aspects of a population pharmacodynamic model for cognitive effects in Alzheimer patients treated with tacrine.

Results and validation of a population pharmacodynamic model for cognitive effects in Alzheimer patients treated with tacrine.

Borna disease virus, a negative-strand RNA virus, transcribes in the nucleus of infected cells.

Brain proteins in plants: an Arabidopsis homolog to neurotransmitter pathway activators is part of a DNA binding complex.

Pur-1, a zinc-finger protein that binds to purine-rich sequences, transactivates an insulin promoter in heterologous cells.

Poly(L-alanine) as a universal reference material for understanding protein energies and structures.

Tumor necrosis factor alpha response elements in the HLA-DRA promoter: identification of a tumor necrosis factor alpha-induced DNA-protein complex in astrocytes.

DNA rearrangement causes hepatocarcinogenesis in albumin-plasminogen activator transgenic mice.

Stable heteroplasmy for a large-scale deletion in the coding region of Drosophila subobscura mitochondrial DNA.

The calorically restricted low-fat nutrient-dense diet in Biosphere 2 significantly lowers blood glucose, total leukocyte count, cholesterol, and blood pressure in humans.

Activation of estrogen receptor transfected into a receptor-negative breast cancer cell line decreases the metastatic and invasive potential of the cells.

Morphological changes in liposomes caused by polymerization of encapsulated actin and spontaneous formation of actin bundles.

Similarity between fluorescein-specific T-cell receptor and antibody in chemical details of antigen recognition.

Phosphorylation state and DNA-binding activity of c-Jun depend on the intracellular concentration of binding sites.

Glutathione deficiency increases hepatic ascorbic acid synthesis in adult mice.

Calcium-myristoyl protein switch.

Characterization by yeast artificial chromosome cloning of the linked apolipoprotein(a) and plasminogen genes and identification of the apolipoprotein(a) 5' flanking region.

Dominant genetics using a yeast genomic library under the control of a strong inducible promoter.

Purification of human leukotriene C4 synthase.

Transcription-dependent and transcription-independent nucleosome disruption induced by dioxin.

Characterization of the tumor suppressor protein p53 as a protein kinase C substrate and a S100b-binding protein.

Subpicosecond equilibration of excitation energy in isolated photosystem II reaction centers.

Detection of dystrophin in the postsynaptic density of rat brain and deficiency in a mouse model of Duchenne muscular dystrophy.

Year: 1992 Volume: 89 Issue: 22

Year: 1992 Volume: 89 Issue: 21

Year: 1992 Volume: 89 Issue: 20

Year: 1992 Volume: 89 Issue: 19

Year: 1992 Volume: 89 Issue: 18

Year: 1992 Volume: 89 Issue: 17

Year: 1992 Volume: 89 Issue: 16

Year: 1992 Volume: 89 Issue: 15

Year: 1992 Volume: 89 Issue: 14

Year: 1992 Volume: 89 Issue: 13

Year: 1992 Volume: 89 Issue: 12

Year: 1992 Volume: 89 Issue: 11

Year: 1992 Volume: 89 Issue: 10

Year: 1992 Volume: 89 Issue: 9

Year: 1992 Volume: 89 Issue: 8

Year: 1992 Volume: 89 Issue: 7

Year: 1992 Volume: 89 Issue: 6

Year: 1992 Volume: 89 Issue: 5

Year: 1992 Volume: 89 Issue: 4

Year: 1992 Volume: 89 Issue: 3

Year: 1992 Volume: 89 Issue: 2

Year: 1992 Volume: 89 Issue: 1

On the nature of global classification.

Theory of hydrophobicity: transient cavities in molecular liquids.

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Purification of human leukotriene C4 synthase.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Soberman, R. J. Myers, P. Penrose, J. F. Goppelt-struebe, M. Jack, R. M. Gagnon, L. Lam, B. K. Austen, K. F.
Description	Author Affiliation: Penrose JF ( Department of Rheumatology and Immunology, Brigham and Women's Hospital, Boston, MA.);
Abstract	Leukotriene (LT) C4 synthase, the enzyme that catalyzes the conjugation of LTA4 with reduced glutathione to form LTC4, was purified to homogeneity from the KG-1 myeloid cell line after solubilization of the microsomes utilizing a combination of 0.4% sodium deoxycholate and 0.4% Triton X-102. The solubilized enzyme was then applied to an S-hexyl-glutathione-agarose column that was eluted by the use of 7.5 mM probenecid. After removal of the probenecid by sequential concentration and dilution in an Amicon concentrator, the enzyme was additionally purified and concentrated by binding to and elution from approximately 75 mg of S-hexyl-glutathione-agarose. The enzyme was further resolved by electrophoresis with a nondenaturing Tris-glycine gel, and the LTC4 synthase activity was localized to slices 3 and 4. When the remainder of the eluate from the nondenaturing gel was precipitated by acetone and analyzed by 14% SDS/PAGE with silver staining, a single protein band of 18 kDa was associated with LTC4 synthase activity and was not present in the eluates of slices lacking activity. The overall recovery was 12.5%. In a separate preliminary purification, in which the yield was only approximately 1%, the eluates of the nondenaturing gel had also revealed a single protein of 18 kDa by SDS/PAGE, which was present only in the eluates with LTC4 synthase activity. These data identify LTC4 synthase as a protein of 18 kDa, a size consistent with its membership in the microsomal glutathione S-transferase family.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	23
Volume Number	89
Language	English
Publisher	National Academy of Sciences
Publisher Date	1992-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Glutathione Transferase Isolation & Purification Metabolism Chromatography, Affinity Glutathione In Vitro Techniques Microsomes Enzymology Molecular Weight Probenecid Pharmacology Tumor Cells, Cultured Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in