NDLI: mu opiate receptor: cDNA cloning and expression.

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mu opiate receptor: cDNA cloning and expression.

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mu opiate receptor: cDNA cloning and expression.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Uhl, G. R. Imai, Y. Wang, J. B. Eppler, C. M. Gregor, P. Spivak, C. E.
Description	Author Affiliation: Wang JB ( Molecular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD.);
Abstract	mu opiate receptors recognize morphine with high affinity. A 2.1-kb rat brain cDNA whose predicted translation product displays 63% identity with recently described delta and kappa opiate receptor sequences was identified through polymerase chain reaction and cDNA homology approaches. This cDNA recognizes a 10.5-kb mRNA that is expressed in thalamic neurons. COS-cell expression confers naloxonazine-, Na(+)-, and GTP-sensitive binding of mu but not delta or kappa opioid ligands. Expressing cells bind morphine, [D-Ala2,N-methyl-Phe4,glyol5]enkephalin (DAMGO), and [D-Ala2,D-Leu5]enkephalin (DADLE) with nanomolar or subnanomolar affinities, defining a mu opiate receptor that avidly recognizes analgesic and euphoric opiate drugs and opioid peptides.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	21
Volume Number	90
Language	English
Publisher	National Academy of Sciences
Publisher Date	1993-12-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Brain Metabolism DNA, Complementary Receptors, Opioid, Mu Biosynthesis Amino Acid Sequence Animals Cloning, Molecular DNA Primers Chemistry Enkephalin, Ala(2)-MePhe(4)-Gly(5)- Enkephalin, Leucine-2-Alanine Enkephalins Escherichia Coli Gene Expression Kinetics Molecular Sequence Data Polymerase Chain Reaction Protein Biosynthesis RNA, Messenger Isolation & Purification Recombinant Proteins Sequence Homology, Amino Acid Comparative Study Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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