NDLI: Bacteriophage T7 gene 2.5 protein: an essential protein for DNA replication.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1993 Volume: 90 Issue: 24

Year: 1993 Volume: 90 Issue: 23

Year: 1993 Volume: 90 Issue: 22

Year: 1993 Volume: 90 Issue: 21

Overview of imaging science.

Charged-coupled detector sky surveys.

Pattern recognition at the Fermilab collider and Superconducting Supercollider.

The intelligent camera: images of computer vision.

Shape and motion from image streams: a factorization method.

UDPglucose: fatty acid transglucosylation and transacylation in triacylglucose biosynthesis.

Spinons and holons for the one-dimensional three-band Hubbard models of high-temperature superconductors.

Sex pheromone for the brownbanded cockroach is an unusual dialkyl-substituted alpha-pyrone.

Capelli's theory, Koszul maps, and superalgebras.

High-affinity self-reactive human antibodies by design and selection: targeting the integrin ligand binding site.

Stoichiometry and structure of complexes of DNA oligomers with microgonotropens and distamycin by 1H NMR spectroscopy and molecular modeling.

Diversified sequences of peptide epitope for same-RNA recognition.

Neurologic disease induced in transgenic mice by cerebral overexpression of interleukin 6.

Modulation of transmission gain by protons at the photoreceptor output synapse.

Identification of an embryonic isoform of myelin basic protein that is expressed widely in the mouse embryo.

Role of nitric oxide in the control of luteinizing hormone-releasing hormone release in vivo and in vitro.

Voltage-dependent potentiation of the activity of cardiac L-type calcium channel alpha 1 subunits due to phosphorylation by cAMP-dependent protein kinase.

Multiple integrins mediate cell attachment to cytotactin/tenascin.

Ceramide UDPgalactosyltransferase from myelinating rat brain: purification, cloning, and expression.

Direct identification of an endogenous peptide recognized by multiple HLA-A2.1-specific cytotoxic T cells.

The Src homology 2 domain of the protein-tyrosine kinase p56lck mediates both intermolecular and intramolecular interactions.

Identification of a specific region of low molecular weight phospholipases A2 (residues 21-40) as a potential target for structure-based design of inhibitors of these enzymes.

Protein kinase A antagonizes platelet-derived growth factor-induced signaling by mitogen-activated protein kinase in human arterial smooth muscle cells.

Increasing cAMP attenuates activation of mitogen-activated protein kinase.

Transforming growth factor beta effects on expression of G1 cyclins and cyclin-dependent protein kinases.

The Fas protein is expressed at high levels on CD4+CD8+ thymocytes and activated mature lymphocytes in normal mice but not in the lupus-prone strain, MRL lpr/lpr.

Immunosuppressant FK506 enhances phosphorylation of nitric oxide synthase and protects against glutamate neurotoxicity.

Brain-specific tropomyosins TMBr-1 and TMBr-3 have distinct patterns of expression during development and in adult brain.

Cell-free pool of CD14 mediates activation of transcription factor NF-kappa B by lipopolysaccharide in human endothelial cells.

Glycosyl-phosphatidylinositol-anchored or integral membrane forms of CD14 mediate identical cellular responses to endotoxin.

Lipopolysaccharide-binding protein as a major plasma protein responsible for endotoxemic shock.

Functional roles of the nucleotide-binding folds in the activation of the cystic fibrosis transmembrane conductance regulator.

Interleukin 12 acts directly on CD4+ T cells to enhance priming for interferon gamma production and diminishes interleukin 4 inhibition of such priming.

Protonation states of membrane-embedded carboxylic acid groups in rhodopsin and metarhodopsin II: a Fourier-transform infrared spectroscopy study of site-directed mutants.

Modulation of learning, pain thresholds, and thermoregulation in the rat by preparations of free purified alpha-linolenic and linoleic acids: determination of the optimal omega 3-to-omega 6 ratio.

Selective erythroid replacement in murine beta-thalassemia using fetal hematopoietic stem cells.

Daidzin and daidzein suppress free-choice ethanol intake by Syrian golden hamsters.

Oligonucleotide clamps arrest DNA synthesis on a single-stranded DNA target.

An unusual active hexose transport system in human and mouse preimplantation embryos.

Amino acid sequence of a putative sodium channel expressed in the giant axon of the squid Loligo opalescens.

Linking yeast genetics to mammalian genomes: identification and mapping of the human homolog of CDC27 via the expressed sequence tag (EST) data base.

Heterologous expression of specific K+ channels in T lymphocytes: functional consequences for volume regulation.

Selective inhibition of the carotid body sensory response to hypoxia by the substance P receptor antagonist CP-96,345.

The role of complement component C3b and its receptors in sperm-oocyte interaction.

Molecular cloning and functional expression of a cDNA encoding glycosylation-inhibiting factor.

Characterization of a gene encoding a Ca(2+)-ATPase-like protein in the plastid envelope.

Molecular cloning of the human and monkey sperm surface protein PH-20.

Ets oncogene-related gene Elg functions in Drosophila oogenesis.

Phospholipase D-catalyzed hydrolysis of phosphatidylcholine provides the choline precursor for acetylcholine synthesis in a human neuronal cell line.

Determination of mRNA fate by different RNA polymerase II promoters.

Disruption of mitotic spindle orientation in a yeast dynein mutant.

Sequencing using pulsed field and image reconstruction.

A 4.5-megabase yeast artificial chromosome contig from human chromosome 13q14.3 ordering 9 polymorphic microsatellites (22 sequence-tagged sites) tightly linked to the Wilson disease locus.

Identification and characterization of hematopoietic stem cells from the yolk sac of the early mouse embryo.

Serine-173 of the Epstein-Barr virus ZEBRA protein is required for DNA binding and is a target for casein kinase II phosphorylation.

Stimulation of Trypanosoma cruzi adenylyl cyclase by an alpha D-globin fragment from Triatoma hindgut: effect on differentiation of epimastigote to trypomastigote forms.

Probing the role of loop 2 in Ras function with unnatural amino acids.

beta-Amyloid precursor protein binds to the neurite-promoting IKVAV site of laminin.

Blastocyst's state of activity determines the 'window' of implantation in the receptive mouse uterus.

Borrelia burgdorferi is clonal: implications for taxonomy and vaccine development.

Beet western yellows virus-associated RNA: an independently replicating RNA that stimulates virus accumulation.

Bacteriophage T7 gene 2.5 protein: an essential protein for DNA replication.

Allelic instability in mitosis: a unified model for dominant disorders.

Lymphocytes and macrophages outnumber oligodendroglia in normal fish spinal cord.

Transcription factors NF-IL6 and NF-kappa B synergistically activate transcription of the inflammatory cytokines, interleukin 6 and interleukin 8.

On the role of macrophages in anthrax.

Mutant woodchuck hepatitis virus genomes from virions resemble rearranged hepadnaviral integrants in hepatocellular carcinoma.

Control of folding and membrane translocation by binding of the chaperone DnaJ to nascent polypeptides.

Investigation of the validity of species status of Ixodes dammini (Acari: Ixodidae) using rDNA.

Cationic drug analysis using matrix-assisted laser desorption/ionization mass spectrometry: application to influx kinetics, multidrug resistance, and intracellular chemical change.

mu opiate receptor: cDNA cloning and expression.

Atrial G protein-activated K+ channel: expression cloning and molecular properties.

Essential role of hypothalamic muscarinic and alpha-adrenergic receptors in atrial natriuretic peptide release induced by blood volume expansion.

Relationship between NMR transverse relaxation, trabecular bone architecture, and strength.

Inhibition of endosome fusion by phospholipase A2 (PLA2) inhibitors points to a role for PLA2 in endocytosis.

Differential expression of four members of the POU family of proteins in activated and phorbol 12-myristate 13-acetate-treated Jurkat T cells.

Tissue- and stratum-specific expression of the human involucrin promoter in transgenic mice.

An ets-related gene, ERG, is rearranged in human myeloid leukemia with t(16;21) chromosomal translocation.

G-protein alpha o subunit: mutation of conserved cysteines identifies a subunit contact surface and alters GDP affinity.

26-10 Fab-digoxin complex: affinity and specificity due to surface complementarity.

Split decomposition: a technique to analyze viral evolution.

Conformation-sensitive gel electrophoresis for rapid detection of single-base differences in double-stranded PCR products and DNA fragments: evidence for solvent-induced bends in DNA heteroduplexes.

Formation of functional peptide complexes of class II major histocompatibility complex proteins from subunits produced in Escherichia coli.

Fibroblasts can induce thymocyte positive selection in vivo.

An ethoxyquin-inducible aldehyde reductase from rat liver that metabolizes aflatoxin B1 defines a subfamily of aldo-keto reductases.

Arabidopsis thaliana auxotrophs reveal a tryptophan-independent biosynthetic pathway for indole-3-acetic acid.

Cloning of cDNA for the alpha subunit of mouse insulin-like growth factor I receptor and the role of the receptor in metanephric development.

Molecular cloning and characterization of the human A3 adenosine receptor.

The maize transposable element system Ac/Ds as a mutagen in Arabidopsis: identification of an albino mutation induced by Ds insertion.

Mapping of interacting domains between the nucleocapsid protein and the phosphoprotein of vesicular stomatitis virus by using a two-hybrid system.

Central oxytocin inhibition of salt appetite in rats: evidence for differential sensing of plasma sodium and osmolality.

Altered protein binding to the octamer motif appears to be an early event in programmed neuronal cell death.

Salmonella typhimurium induces membrane ruffling by a growth factor-receptor-independent mechanism.

Further evidence for function of the Drosophila Notch protein as a transmembrane receptor.

Formation of layer-specific fiber projections to the hippocampus in vitro.

Structural characterization of the complete human perlecan gene and its promoter.

Notice of retraction.

Nitric oxide: foe or friend to the injured brain?

Images of science: science of images.

Model observers for assessment of image quality.

Image processing: some challenging problems.

Color, contrast sensitivity, and the cone mosaic.

Color appearance: the effects of illumination and spatial pattern.

Nonlinear processes in visual pattern discrimination.

Issues of imaging science for future consideration.

Nitric oxide protects against cellular damage and cytotoxicity from reactive oxygen species.

Subunit structure of a class A aspartate transcarbamoylase from Pseudomonas fluorescens.

Repair by human cell extracts of single (6-4) and cyclobutane thymine-thymine photoproducts in DNA.

Escherichia coli ribosomal protein L7/L12 dimers remain fully active after interchain crosslinking of the C-terminal domains in two orientations.

Gene targeting of X chromosome-linked chronic granulomatous disease locus in a human myeloid leukemia cell line and rescue by expression of recombinant gp91phox.

Local breathing and global unfolding in hydrogen exchange of barnase and its relationship to protein folding pathways.

Use of a gene encoding a suppressor tRNA as a reporter of transcription: analyzing the action of the Nun protein of bacteriophage HK022.

Refined 1.8 A structure of human aldose reductase complexed with the potent inhibitor zopolrestat.

Crystal structure of a bacterial sialidase (from Salmonella typhimurium LT2) shows the same fold as an influenza virus neuraminidase.

Analysis of somatic hypermutation in mouse Peyer's patches using immunoglobulin kappa light-chain transgenes.

Gene sequence tags from Plasmodium falciparum genomic DNA fragments prepared by the 'genease' activity of mung bean nuclease.

Specific prolongation of allograft survival by a T-cell-receptor-derived peptide.

Mutants defective in the energy-conserving NADH dehydrogenase of Salmonella typhimurium identified by a decrease in energy-dependent proteolysis after carbon starvation.

Genomic clone for sandbar shark lambda light chain: generation of diversity in the absence of gene rearrangement.

Occurrence of fragmented 16S rRNA in an obligate bacterial endosymbiont of Paramecium caudatum.

Localized 1H NMR measurement of glucose consumption in the human brain during visual stimulation.

Antisense inhibition of the p65 subunit of NF-kappa B blocks tumorigenicity and causes tumor regression.

Leucine aminopeptidase: an inducible component of the defense response in Lycopersicon esculentum (tomato).

Coding coenzyme handles: a hypothesis for the origin of the genetic code.

Mutations that alter the third cytoplasmic loop of the a-factor receptor lead to a constitutive and hypersensitive phenotype.

Genetic evidence for the roles of the bud-site-selection genes BUD5 and BUD2 in control of the Rsr1p (Bud1p) GTPase in yeast.

Preformed dimeric state of the sensor protein VirA is involved in plant--Agrobacterium signal transduction.

Transforming growth factor beta 1 (TGF-beta 1) controls expression of major histocompatibility genes in the postnatal mouse: aberrant histocompatibility antigen expression in the pathogenesis of the TGF-beta 1 null mouse phenotype.

Three-dimensional model for the hormone binding domains of steroid receptors.

Cloning and pharmacological characterization of a rat kappa opioid receptor.

Formation of the active site of ribulose-1,5-bisphosphate carboxylase/oxygenase by a disorder-order transition from the unactivated to the activated form.

Rapid interchange between two distinct forms of cyanobacterial photosystem II reaction-center protein D1 in response to photoinhibition.

Cell-specific action and mutable structure of a transcription factor effector domain.

Characterization of composite aminodeoxyisochorismate synthase and aminodeoxyisochorismate lyase activities of anthranilate synthase.

D-chiro-inositol metabolism in diabetes mellitus.

Activation by nitric oxide of an oxidative-stress response that defends Escherichia coli against activated macrophages.

Characterization of functional organization within rat barrel cortex using intrinsic signal optical imaging through a thinned skull.

Year: 1993 Volume: 90 Issue: 20

Year: 1993 Volume: 90 Issue: 19

Year: 1993 Volume: 90 Issue: 18

Year: 1993 Volume: 90 Issue: 17

Year: 1993 Volume: 90 Issue: 16

Year: 1993 Volume: 90 Issue: 15

Year: 1993 Volume: 90 Issue: 14

Year: 1993 Volume: 90 Issue: 13

Year: 1993 Volume: 90 Issue: 12

Year: 1993 Volume: 90 Issue: 11

Year: 1993 Volume: 90 Issue: 10

Year: 1993 Volume: 90 Issue: 9

Year: 1993 Volume: 90 Issue: 8

Year: 1993 Volume: 90 Issue: 7

Year: 1993 Volume: 90 Issue: 6

Year: 1993 Volume: 90 Issue: 5

Year: 1993 Volume: 90 Issue: 4

Year: 1993 Volume: 90 Issue: 3

Year: 1993 Volume: 90 Issue: 2

Year: 1993 Volume: 90 Issue: 1

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Bacteriophage T7 gene 2.5 protein: an essential protein for DNA replication.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kim, Y. T. Richardson, C. C.
Description	Author Affiliation: Kim YT ( Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115.);
Abstract	The product of gene 2.5 of bacteriophage T7, a single-stranded DNA binding protein, physically interacts with the phage-encoded gene 5 protein (DNA polymerase) and gene 4 proteins (helicase and primase) and stimulates their activities. Genetic analysis of T7 phage defective in gene 2.5 shows that the gene 2.5 protein is essential for T7 DNA replication and growth. T7 phages that contain null mutants of gene 2.5 were constructed by homologous recombination. These gene 2.5 null mutants contain either a deletion of gene 2.5 (T7 delta 2.5) or an insertion into gene 2.5 and cannot grow in Escherichia coli (efficiency of plating, < 10(-8)). After infection of E. coli with T7 delta 2.5, host DNA synthesis is shut off, and phage DNA synthesis is reduced to < 1% of phage DNA synthesis in wild-type T7-infected E. coli cells as measured by incorporation of [3H]thymidine. In contrast, RNA synthesis is essentially normal in T7 delta 2.5-infected cells. The defects in growth and DNA replication are overcome by wild-type gene 2.5 protein expressed from a plasmid harboring the T7 gene 2.5.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	21
Volume Number	90
Language	English
Publisher	National Academy of Sciences
Publisher Date	1993-12-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Bacteriophage T7 Metabolism DNA Replication Genes, Viral Viral Proteins Genetics Cloning, Molecular DNA Primers Escherichia Coli Kinetics Molecular Sequence Data Plasmids Polymerase Chain Reaction Restriction Mapping Time Factors Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in