NDLI: Opsins with mutations at the site of chromophore attachment constitutively activate transducin but are not phosphorylated by rhodopsin kinase.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1994 Volume: 91 Issue: 26

Year: 1994 Volume: 91 Issue: 26 Suppl

Year: 1994 Volume: 91 Issue: 25

Year: 1994 Volume: 91 Issue: 24

Year: 1994 Volume: 91 Issue: 23

Year: 1994 Volume: 91 Issue: 22

Year: 1994 Volume: 91 Issue: 21

Year: 1994 Volume: 91 Issue: 20

Year: 1994 Volume: 91 Issue: 19

Year: 1994 Volume: 91 Issue: 18

Year: 1994 Volume: 91 Issue: 17

Year: 1994 Volume: 91 Issue: 16

Year: 1994 Volume: 91 Issue: 15

Year: 1994 Volume: 91 Issue: 14

Year: 1994 Volume: 91 Issue: 13

Year: 1994 Volume: 91 Issue: 12

Resource edibility and trophic exploitation in an old-field food web.

Activation of JAK2 tyrosine kinase by prolactin receptors in Nb2 cells and mouse mammary gland explants.

Reaction of diphtheria toxin channels with sulfhydryl-specific reagents: observation of chemical reactions at the single molecule level.

Role of scatter factor in the pathogenesis of AIDS-related Kaposi sarcoma.

p56lck-independent activation and tyrosine phosphorylation of p72syk by T-cell antigen receptor/CD3 stimulation.

Expression of cytokeratin confers multiple drug resistance.

Bicarbonate conductance and pH regulatory capability of cystic fibrosis transmembrane conductance regulator.

A conserved heptamer motif for ribosomal RNA transcription termination in animal mitochondria.

Calcineurin is essential in cyclosporin A- and FK506-sensitive yeast strains.

Two distinct signaling pathways trigger the expression of inducible nitric oxide synthase in rat renal mesangial cells.

Estradiol causes the rapid accumulation of cAMP in human prostate.

Analysis of gene expression in the preimplantation mouse embryo: use of mRNA differential display.

Expression and growth inhibitory effect of decapentaplegic Vg-related protein 6: evidence for a regulatory role in keratinocyte differentiation.

Heterogeneity of T-cell receptor alpha-chain complementarity-determining region 3 in myelin basic protein-specific T cells increases with severity of multiple sclerosis.

Anti-peptidyl transferase leader peptides of attenuation-regulated chloramphenicol-resistance genes.

Inhibition of retinoic acid-induced activation of 3' human HOXB genes by antisense oligonucleotides affects sequential activation of genes located upstream in the four HOX clusters.

Low-dose interleukin 2 prevents the development of Epstein-Barr virus (EBV)-associated lymphoproliferative disease in scid/scid mice reconstituted i.p. with EBV-seropositive human peripheral blood lymphocytes.

Molecular cloning of fibroblast activation protein alpha, a member of the serine protease family selectively expressed in stromal fibroblasts of epithelial cancers.

Prion isolate specified allotypic interactions between the cellular and scrapie prion proteins in congenic and transgenic mice.

Naive idiotype-specific CD4+ T cells and immunosurveillance of B-cell tumors.

D1 dopamine receptor immunoreactivity in human and monkey cerebral cortex: predominant and extrasynaptic localization in dendritic spines.

A rosy future for heterochromatin.

Genetic instability of plant tissue cultures: breakdown of normal controls.

Human giant cell tumors of the bone (osteoclastomas) are estrogen target cells.

Molecular cloning of an essential subunit of RNA polymerase II elongation factor SIII.

The cooperativity and allosteric inhibition of Escherichia coli phosphofructokinase depend on the interaction between threonine-125 and ATP.

Herpes simplex virus 1 gamma(1)34.5 gene function, which blocks the host response to infection, maps in the homologous domain of the genes expressed during growth arrest and DNA damage.

Platelet-activating factor and retinoic acid synergistically activate the inducible prostaglandin synthase gene.

Circadian expression of the luciferin-binding protein correlates with the binding of a protein to the 3' untranslated region of its mRNA.

Angular orientation of the stable tyrosyl radical within photosystem II by high-field 245-GHz electron paramagnetic resonance.

Exo-endocytosis and closing of the fission pore during endocytosis in single pituitary nerve terminals internally perfused with high calcium concentrations.

Linker DNA accessibility in chromatin fibers of different conformations: a reevaluation.

Epithelial differentiation of metanephric mesenchymal cells after stimulation with hepatocyte growth factor or embryonic spinal cord.

A cell cycle-regulated inhibitor of cyclin-dependent kinases.

Mouse stem cell antigen Sca-2 is a member of the Ly-6 family of cell surface proteins.

The LW blood group glycoprotein is homologous to intercellular adhesion molecules.

Fast photoinduced electron transfer through DNA intercalation.

p53-dependent G1 arrest involves pRB-related proteins and is disrupted by the human papillomavirus 16 E7 oncoprotein.

BST-1, a surface molecule of bone marrow stromal cell lines that facilitates pre-B-cell growth.

Terminal neuroendocrine differentiation of human prostate carcinoma cells in response to increased intracellular cyclic AMP.

Role of Src homology 3 domains in assembly and activation of the phagocyte NADPH oxidase.

Disulfide cross-linking in crude embryonic lysates reveals three complexes of the Drosophila morphogen dorsal and its inhibitor cactus.

Glutamine repeats as polar zippers: their possible role in inherited neurodegenerative diseases.

Astrocytosis and axonal proliferation in the hippocampus of S100b transgenic mice.

Island rescue PCR: a rapid and efficient method for isolating transcribed sequences from yeast artificial chromosomes and cosmids.

Pregnancy-related steroids are potential negative regulators of B lymphopoiesis.

Insulin promotes the biosynthesis and secretion of apolipoprotein B-48 by altering apolipoprotein B mRNA editing.

Induction of cytochrome CYPIA1 and formation of toxic metabolites of benzo[a]pyrene by rat aorta: a possible role in atherogenesis.

Mapping of herpes simplex virus 1 genes with mutations which overcome host restrictions to infection.

Opsins with mutations at the site of chromophore attachment constitutively activate transducin but are not phosphorylated by rhodopsin kinase.

Activation of the pp90rsk and mitogen-activated serine/threonine protein kinases by ionizing radiation.

Properties of permease dimer, a fusion protein containing two lactose permease molecules from Escherichia coli.

Luminescent oxygen channeling immunoassay: measurement of particle binding kinetics by chemiluminescence.

The cytoplasmic domain of the Na+/H+ exchangers (NHEs) dictates the nature of the hormonal response: behavior of a chimeric human NHE1/trout beta NHE antiporter.

Limb and lower-body duplications induced by retinoic acid in mice.

Methane production in terrestrial arthropods.

A role for destabilizing amino acid replacements in light-chain amyloidosis.

Cell fates in leech embryos with duplicated lineages.

A net +1 frameshift permits synthesis of thymidine kinase from a drug-resistant herpes simplex virus mutant.

Extraordinarily polymorphic microsatellite DNA in barley: species diversity, chromosomal locations, and population dynamics.

Spectrophotometric assay for enzyme-mediated unwinding of double-stranded DNA.

The bat gene of Halobacterium halobium encodes a trans-acting oxygen inducibility factor.

Evidence that inactive p42 mitogen-activated protein kinase and inactive Rsk exist as a heterodimer in vivo.

Liberation of an interaction domain from the phosphotransfer region of CheA, a signaling kinase of Escherichia coli.

Structure and cell cycle-regulated transcription of the human cyclin A gene.

Female brain size and parental care in carnivores.

Senescence of immortal human fibroblasts by the introduction of normal human chromosome 6.

Suppression of Myc, but not E1a, transformation activity by Max-associated proteins, Mad and Mxi1.

Stable DNA transformation in the obligate intracellular parasite Toxoplasma gondii by complementation of tryptophan auxotrophy.

Three tumor-suppressor regions on chromosome 11p identified by high-resolution deletion mapping in human non-small-cell lung cancer.

Molecular mapping of the heparin-binding exosite of thrombin.

MYCN is retained in single copy at chromosome 2 band p23-24 during amplification in human neuroblastoma cells.

Immortalized germ cells undergo meiosis in vitro.

Identification of plant genetic loci involved in a posttranscriptional mechanism for meiotically reversible transgene silencing.

Alterations in major histocompatibility complex association of myocarditis induced by coxsackievirus B3 mutants selected with monoclonal antibodies to group A streptococci.

Generation of a fusion partner to sample the repertoire of splenic B cells destined for apoptosis.

Selective inactivation of viruses in the presence of human platelets: UV sensitization with psoralen derivatives.

Human immunodeficiency virus type 1 may preferentially integrate into chromatin occupied by L1Hs repetitive elements.

Role of abnormally phosphorylated tau in the breakdown of microtubules in Alzheimer disease.

Defective muscle basement membrane and lack of M-laminin in the dystrophic dy/dy mouse.

Structural and mechanistic characteristics of dihydropteridine reductase: a member of the Tyr-(Xaa)3-Lys-containing family of reductases and dehydrogenases.

Tyrosine kinase-deficient mutant human insulin receptors (Met1153-->Ile) overexpressed in transfected rat adipose cells fail to mediate translocation of epitope-tagged GLUT4.

Granulocyte/macrophage colony-stimulating factor-deficient mice show no major perturbation of hematopoiesis but develop a characteristic pulmonary pathology.

Selection of multiple human immunodeficiency virus type 1 variants that encode viral proteases with decreased sensitivity to an inhibitor of the viral protease.

Human serum amyloid P component is an invariant constituent of amyloid deposits and has a uniquely homogeneous glycostructure.

Cloning and expression of a cDNA encoding a brain-specific Na(+)-dependent inorganic phosphate cotransporter.

Proton conduction within the reaction centers of Rhodobacter capsulatus: the electrostatic role of the protein.

Spatial distribution of two maternal messengers in Paracentrotus lividus during oogenesis and embryogenesis.

Specific T-cell tolerance may be preceded by a primary response.

A synthetic retinoid antagonist inhibits the human immunodeficiency virus type 1 promoter.

The animal model of human amnesia: long-term memory impaired and short-term memory intact.

Expansion of CREB's DNA recognition specificity by Tax results from interaction with Ala-Ala-Arg at positions 282-284 near the conserved DNA-binding domain of CREB.

Targeted mutation of the CREB gene: compensation within the CREB/ATF family of transcription factors.

Finding a needle in a haystack: detection of a small protein (the 12-kDa VP26) in a large complex (the 200-MDa capsid of herpes simplex virus).

D-MEF2: a MADS box transcription factor expressed in differentiating mesoderm and muscle cell lineages during Drosophila embryogenesis.

Differences in dorsal and ventral pigmentation result from regional expression of the mouse agouti gene.

Cisplatin-DNA adducts are molecular decoys for the ribosomal RNA transcription factor hUBF (human upstream binding factor).

Transcriptional down-regulation by insulin of the beta 3-adrenergic receptor expression in 3T3-F442A adipocytes: a mechanism for repressing the cAMP signaling pathway.

Dynamic instability of microtubules as an efficient way to search in space.

Disruption of the Golgi apparatus by brefeldin A blocks cell polarization and inhibits directed cell migration.

Effective amplification of long targets from cloned inserts and human genomic DNA.

Macromolecular permeability across the blood-nerve and blood-brain barriers.

Advanced Maillard reaction end products are associated with Alzheimer disease pathology.

Neurally expressed Drosophila genes encoding homologs of the NSF and SNAP secretory proteins.

Membrane repolarization stops caffeine-induced Ca2+ release in skeletal muscle cells.

rbcL gene sequences provide evidence for the evolutionary lineages of leptosporangiate ferns.

Year: 1994 Volume: 91 Issue: 11

Year: 1994 Volume: 91 Issue: 10

Year: 1994 Volume: 91 Issue: 9

Year: 1994 Volume: 91 Issue: 8

Year: 1994 Volume: 91 Issue: 7

Year: 1994 Volume: 91 Issue: 6

Year: 1994 Volume: 91 Issue: 5

Year: 1994 Volume: 91 Issue: 4

Year: 1994 Volume: 91 Issue: 3

Year: 1994 Volume: 91 Issue: 2

Year: 1994 Volume: 91 Issue: 1

Impact melting of frozen oceans on the early Earth: Implications for the origin of life

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Opsins with mutations at the site of chromophore attachment constitutively activate transducin but are not phosphorylated by rhodopsin kinase.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Robinson, P. R. Ohguro, H. Buczylko, J. Palczewski, K.
Description	Author Affiliation: Robinson PR ( Department of Biological Sciences, University of Maryland Baltimore County, Baltimore 21228.);
Abstract	More than 70 mutations in the gene encoding the visual pigment rhodopsin have been identified in patients with autosomal dominant retinitis pigmentosa. Most of these mutations are thought to interfere with proper folding of the membrane protein. However, families with a severe phenotype of retinitis pigmentosa have been identified and shown to carry a mutation at the site of chromophore attachment, Lys-296. This mutation disrupts the inactive conformation of opsin and results in a constitutively active protein that can activate the rod-specific GTP-binding protein, transducin, in the absence of light and in the absence of the chromophore 11-cis-retinal. It has been suggested that this mutant opsin molecule may cause rod degeneration by depletion of the components used to inactivate rhodopsin, such as rhodopsin kinase. In this work we test this idea by determining whether two constitutively active opsin mutants are phosphorylated by rhodopsin kinase. We found that opsin mutants where Lys-296 is replaced either by Glu (K296E) or by Gly (K296G) are not substrates of rhodopsin kinase in the absence of chromophore. However, when K296G is regenerated with a Schiff base complex of 11-cis-retinal and n-propylamine and exposed to illumination, phosphorylation of opsin occurs. These experiments suggest that in the rod photoreceptors of patients with retinitis pigmentosa carrying a mutation at Lys-296, there is persistent activation of the GTP-binding protein-mediated cascade. This may result in a situation that mimics long-term exposure to continuous illumination and results in the degeneration of photoreceptors.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	12
Volume Number	91
Language	English
Publisher	National Academy of Sciences
Publisher Date	1994-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Eye Proteins Protein Kinases Genetics Rod Opsins Metabolism Transducin Amino Acid Sequence Animals Cell Line Cell Membrane Cercopithecus Aethiops G-Protein-Coupled Receptor Kinase 1 Molecular Sequence Data Mutagenesis, Site-Directed Mutation Peptides Chemistry Phosphoproteins Phosphorylation Protein Conformation Recombinant Proteins Structure-Activity Relationship Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in