NDLI: Phosphodiesterase activation by photoexcited rhodopsin is quenched when rhodopsin is phosphorylated and binds the intrinsic 48-kDa protein of rod outer segments.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1986 Volume: 83 Issue: 24

Year: 1986 Volume: 83 Issue: 23

Year: 1986 Volume: 83 Issue: 22

Year: 1986 Volume: 83 Issue: 21

Year: 1986 Volume: 83 Issue: 20

Year: 1986 Volume: 83 Issue: 19

Year: 1986 Volume: 83 Issue: 18

Year: 1986 Volume: 83 Issue: 17

Year: 1986 Volume: 83 Issue: 16

Year: 1986 Volume: 83 Issue: 15

Year: 1986 Volume: 83 Issue: 14

Year: 1986 Volume: 83 Issue: 13

Year: 1986 Volume: 83 Issue: 12

Year: 1986 Volume: 83 Issue: 11

Year: 1986 Volume: 83 Issue: 10

Year: 1986 Volume: 83 Issue: 9

Year: 1986 Volume: 83 Issue: 8

Year: 1986 Volume: 83 Issue: 7

Year: 1986 Volume: 83 Issue: 6

Year: 1986 Volume: 83 Issue: 5

Intermediate filaments in alpha-keratins.

Primary structure of the human melanoma-associated antigen p97 (melanotransferrin) deduced from the mRNA sequence.

Speculations on the early course of evolution.

Molecular cloning of the mouse cell adhesion molecule uvomorulin: cDNA contains a B1-related sequence.

Evolutionary diversity of eukaryotic small-subunit rRNA genes.

Human protein HC and its IgA complex are inhibitors of neutrophil chemotaxis.

Dihydropyridine-sensitive Ca2+ channels in mammalian skeletal muscle cells in culture: electrophysiological properties and interactions with Ca2+ channel activator (Bay K8644) and inhibitor (PN 200-110).

Molecular basis for heterosis for myelin basic protein content in mice.

Intrahepatic transport and utilization of biliary glutathione and its metabolites.

Hybrid hybridoma producing a bispecific monoclonal antibody that can focus effector T-cell activity.

Permissive effect of dexamethasone on the increase of proenkephalin mRNA induced by depolarization of chromaffin cells.

Molecular cloning and DNA sequence of the Arabidopsis thaliana alcohol dehydrogenase gene.

Lateral diffusion of specific antibodies bound to lipid monolayers on alkylated substrates.

Phosphodiesterase activation by photoexcited rhodopsin is quenched when rhodopsin is phosphorylated and binds the intrinsic 48-kDa protein of rod outer segments.

Construction of heterodimer tyrosyl-tRNA synthetase shows tRNATyr interacts with both subunits.

Direct comparison of Ca2+ requirements for calmodulin interaction with and activation of protein phosphatase.

Cloning of the Escherichia coli gene for primosomal protein i: the relationship to dnaT, essential for chromosomal DNA replication.

Intracellular activation of protein kinase C and regulation of the surface transferrin receptor by diacylglycerol is a spontaneously reversible process that is associated with rapid formation of phosphatidic acid.

Studies of the radical species in compound ES of cytochrome c peroxidase altered by site-directed mutagenesis.

Gyration is required for 5S RNA transcription from a chromatin template.

Structure of exotoxin A of Pseudomonas aeruginosa at 3.0-Angstrom resolution.

Isotope-detected 1H NMR studies of proteins: a general strategy for editing interproton nuclear Overhauser effects by heteronuclear decoupling, with application to phage lambda repressor.

Direct measurements of intramolecular electron transfer rates between cytochrome c and cytochrome c peroxidase: effects of exothermicity and primary sequence on rate.

Erythrocyte-neutrophil interactions: formation of leukotriene B4 by transcellular biosynthesis.

Mutations of the Drosophila myosin heavy-chain gene: effects on transcription, myosin accumulation, and muscle function.

The nerve growth factor receptor gene is at human chromosome region 17q12-17q22, distal to the chromosome 17 breakpoint in acute leukemias.

Evidence the yeast STE3 gene encodes a receptor for the peptide pheromone a factor: gene sequence and implications for the structure of the presumed receptor.

Monoclonal antibodies specific for H-2K and H-2D antigens on cytotoxic T cells can inhibit their function.

Biogenesis of the platelet receptor for fibrinogen: evidence for separate precursors for glycoproteins IIb and IIIa.

Autoradiographic comparison of the distribution of the neutral endopeptidase 'enkephalinase' and of mu and delta opioid receptors in rat brain.

Immunoreactivity for a calmodulin-dependent protein kinase is selectively increased in macaque striate cortex after monocular deprivation.

Deduced primary structure of the alpha subunit of the GTP-binding stimulatory protein of adenylate cyclase.

Structure-activity relationship in vinculin: an IR/attenuated total reflection spectroscopic and film balance study.

Low density lipoprotein receptor-dependent prostaglandin synthesis in Swiss 3T3 cells stimulated by platelet-derived growth factor.

Genetic changeover in Drosophila populations.

Frequent lambda light chain gene rearrangement and expression in a Ly-1 B lymphoma with a productive kappa chain allele.

Only high-affinity receptors for interleukin 2 mediate internalization of ligand.

Specificity and mechanism of protein kinase C activation by sn-1,2-diacylglycerols.

Molecular cloning of human ornithine aminotransferase mRNA.

Cytoplasmic synthesis of globin RNA in differentiated murine erythroleukemia cells: possible involvement of RNA-dependent RNA polymerase.

Earthworms produce a collagen-like substance detected by the garter snake vomeronasal system.

Fate of reversing factor during restoration of protein synthesis by hemin or GTP in heme-deficient reticulocyte lysates.

Two acyl-coenzyme A oxidases in peroxisomes of the yeast Candida tropicalis: primary structures deduced from genomic DNA sequence.

Rapid formation of an anion-sensitive active site in stoichiometric complexes of streptokinase and human [Glu1]plasminogen.

The primary structure of rat parathymosin.

A mammalian high mobility group protein recognizes any stretch of six A.T base pairs in duplex DNA.

Functions of and interactions between the A and B blocks in adenovirus type 2-specific VARNA1 gene.

Poly(riboadenylic acid) preferentially inhibits in vitro translation of cellular mRNAs compared with vaccinia virus mRNAs: possible role in vaccinia virus cytopathology.

Isolation of cDNA clones coding for the catalytic subunit of mouse cAMP-dependent protein kinase.

Metastable photoproducts from carbon monoxide myoglobin.

Bryostatin, an activator of the calcium phospholipid-dependent protein kinase, blocks phorbol ester-induced differentiation of human promyelocytic leukemia cells HL-60.

Adhesion and erythrophagocytosis of human senescent erythrocytes by autologous monocytes and their inhibition by beta-galactosyl derivatives.

A developmentally stable chromatin structure in the human beta-globin gene cluster.

The orangutan adult alpha-globin gene locus: duplicated functional genes and a newly detected member of the primate alpha-globin gene family.

Statistical considerations for linkage analysis using recombinant inbred strains and backcrosses.

Microinjection of partially purified protein factor restores DNA damage specifically in group A of xeroderma pigmentosum cells.

Relationship of drinking water disinfectants to plasma cholesterol and thyroid hormone levels in experimental studies.

Selenium and vitamin E inhibit radiogenic and chemically induced transformation in vitro via different mechanisms.

Gated currents generate single spike activity in amacrine cells of the tiger salamander retina.

Heat shock causes destabilization of specific mRNAs and destruction of endoplasmic reticulum in barley aleurone cells.

The genome of frog erythrocytes displays centuplicate replications.

Complete sequence of HLA-B27 cDNA identified through the characterization of structural markers unique to the HLA-A, -B, and -C allelic series.

Cloning of two genes that are specifically expressed in activated cytotoxic T lymphocytes.

Four virally determined nuclear antigens are expressed in Epstein-Barr virus-transformed cells.

Proteins of Escherichia coli come in sizes that are multiples of 14 kDa: domain concepts and evolutionary implications.

Mutations activating human c-Ha-ras1 protooncogene (HRAS1) induced by chemical carcinogens and depurination.

Specific inhibition of mRNA translation by complementary oligonucleotides covalently linked to intercalating agents.

Processing and fatty acid acylation of RAS1 and RAS2 proteins in Saccharomyces cerevisiae.

Identification of yeast mutants with altered telomere structure.

Mutational and in vivo methylation analysis of F-factor PifC protein binding to the pif operator and the region containing the primary origin of mini-F replication.

Induction of c-myc expression in human B lymphocytes by B-cell growth factor and anti-immunoglobulin.

Isolation of a cDNA clone encoding the amino-terminal region of human apolipoprotein B.

Altered expression of G1-specific genes in human malignant myeloid cells.

Bipotential precursors of putative fibrous astrocytes and oligodendrocytes in rat cerebellar cultures express distinct surface features and 'neuron-like' gamma-aminobutyric acid transport.

Melanin-concentrating hormone: unique peptide neuronal system in the rat brain and pituitary gland.

Year: 1986 Volume: 83 Issue: 4

Year: 1986 Volume: 83 Issue: 3

Year: 1986 Volume: 83 Issue: 2

Year: 1986 Volume: 83 Issue: 1

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Phosphodiesterase activation by photoexcited rhodopsin is quenched when rhodopsin is phosphorylated and binds the intrinsic 48-kDa protein of rod outer segments.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kühn, H. Wilden, U. Hall, S. W.
Abstract	Each photoexcited rhodopsin (R) molecule catalyzes binding of GTP to many copies of the guanine nucleotide-binding protein transducin, which, in its GTP-binding form, then activates cGMP phosphodiesterase (PDEase). Subsequent deactivation of this light-activated enzyme cascade involves hydrolysis of the GTP bound to transducin, as well as decay of the activating capacity of R. We report here that deactivation of PDEase in rod outer segment suspensions is highly enhanced by addition of ATP and purified 48-kDa protein, which is an intrinsic rod outer segment protein that is soluble in the dark but binds to photolyzed rhodopsin that has been phosphorylated by rhodopsin kinase and ATP [Kühn, H., Hall, S.W. & Wilden, U. (1984) FEBS Lett. 176, 473-478]. To analyze the mechanism by which ATP and 48-kDa protein deactivate PDEase, we used an ATP-free system consisting of thoroughly washed disk membranes, whose rhodopsin had been previously phosphorylated and chromophore-regenerated, and to which purified PDEase and transducin were reassociated. Such phosphorylated membranes exhibited a significantly lower (by a factor less than or equal to 5) light-induced PDEase-activating capacity than unphosphorylated controls. Addition of purified 48-kDa protein to phosphorylated membranes further suppressed their PDEase-activating capacity; suppression could be as high as 98% (as compared to unphosphorylated membranes), depending on the amount of 48-kDa protein and the flash intensity. Addition of ATP had little further effect. In contrast, PDEase activation or deactivation with unphosphorylated control membranes was not influenced by 48-kDa protein, even in the presence of ATP, provided rhodopsin kinase was absent. Our data suggest that 48-kDa protein binds to phosphorylated R* and thereby quenches its capacity to activate transducin and PDEase.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	5
Volume Number	83
Language	English
Publisher	National Academy of Sciences
Publisher Date	1986-04-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	3',5'-Cyclic-GMP Phosphodiesterases Metabolism Phosphoproteins Physiology Photoreceptor Cells Retinal Pigments Rhodopsin Rod Cell Outer Segment Vision, Ocular Adenosine Triphosphate Animals Enzyme Activation Membrane Proteins Molecular Weight Radiation Effects Transducin Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in