NDLI: Targeted DNA recombination in vivo using an adenovirus carrying the cre recombinase gene.

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Earthquake prediction: the scientific challenge.

Earthquake prediction: the interaction of public policy and science.

Initiation process of earthquakes and its implications for seismic hazard reduction strategy.

Intermediate- and long-term earthquake prediction.

Scale dependence in earthquake phenomena and its relevance to earthquake prediction.

Intermediate-term earthquake prediction.

A selective phenomenology of the seismicity of Southern California.

The repetition of large-earthquake ruptures.

Hypothesis testing and earthquake prediction.

What electrical measurements can say about changes in fault systems.

Geochemical challenge to earthquake prediction.

Implications of fault constitutive properties for earthquake prediction.

Nonuniformity of the constitutive law parameters for shear rupture and quasistatic nucleation to dynamic rupture: a physical model of earthquake generation processes.

Rock friction and its implications for earthquake prediction examined via models of Parkfield earthquakes.

Slip complexity in earthquake fault models.

Dynamic friction and the origin of the complexity of earthquake sources.

Slip complexity in dynamic models of earthquake faults.

The organization of seismicity on fault networks.

Geometric incompatibility in a fault system.

Acridones: a chemically new group of protonophores.

Chloroplast DNA evidence of colonization, adaptive radiation, and hybridization in the evolution of the Macaronesian flora.

Motor learning by field approximation.

The effects of sequence context on DNA curvature.

Organ-specific and Agamous-regulated expression and glycosylation of a pollen tube growth-promoting protein.

Detection of a major gene for resistance to fusiform rust disease in loblolly pine by genomic mapping.

Proteins associated with RNase E in a multicomponent ribonucleolytic complex.

Membrane lipid perturbation modifies the set point of the temperature of heat shock response in yeast.

A mechanism for intergenomic integration: abundance of ribulose bisphosphate carboxylase small-subunit protein influences the translation of the large-subunit mRNA.

Movement of yeast cortical actin cytoskeleton visualized in vivo.

Endothelin 3 promotes neural crest cell proliferation and mediates a vast increase in melanocyte number in culture.

Were bowerbirds part of the New Zealand fauna?

Prevention of cardiovascular and renal pathology of aging by the advanced glycation inhibitor aminoguanidine.

Hippocampal atrophy in recurrent major depression.

A rat genetic map constructed by representational difference analysis markers with suitability for large-scale typing.

POU domain factor Brn-3b is required for the development of a large set of retinal ganglion cells.

Long-time quantum simulation of the primary charge separation in bacterial photosynthesis.

Targeted DNA recombination in vivo using an adenovirus carrying the cre recombinase gene.

A yeast artificial chromosome-based map of the region of chromosome 20 containing the diabetes-susceptibility gene, MODY1, and a myeloid leukemia related gene.

Metalloelastase is required for macrophage-mediated proteolysis and matrix invasion in mice.

Analysis of lipopolysaccharide-response genes in B-lineage cells demonstrates that they can have differentiation stage-restricted expression and contain SH2 domains.

Isolation of 10 differentially expressed cDNAs in p53-induced apoptosis: activation of the vertebrate homologue of the drosophila seven in absentia gene.

Assignment of Rfp-Y to the chicken major histocompatibility complex/NOR microchromosome and evidence for high-frequency recombination associated with the nucleolar organizer region.

STAT3 activation is a critical step in gp130-mediated terminal differentiation and growth arrest of a myeloid cell line.

CNI-1493 inhibits monocyte/macrophage tumor necrosis factor by suppression of translation efficiency.

Human immunodeficiency virus type 1 infection despite prior immunization with a recombinant envelope vaccine regimen.

Axotomized neonatal motoneurons overexpressing the bcl2 proto-oncogene retain functional electrophysiological properties.

The ALIAmide palmitoylethanolamide and cannabinoids, but not anandamide, are protective in a delayed postglutamate paradigm of excitotoxic death in cerebellar granule neurons.

Serotonergic modulation of muscle acetylcholine receptors of different subunit composition.

Absence of opioid stress-induced analgesia in mice lacking beta-endorphin by site-directed mutagenesis.

P-glycoprotein: a major determinant of rifampicin-inducible expression of cytochrome P4503A in mice and humans.

cemA homologue essential to CO2 transport in the cyanobacterium Synechocystis PCC6803.

Chromophore-bearing NH2-terminal domains of phytochromes A and B determine their photosensory specificity and differential light lability.

Electroconvulsive shock and lidocaine reveal rapid consolidation of spatial working memory in the water maze.

Mapping protein-protein interactions by affinity-directed mass spectrometry.

Template-nucleated alanine-lysine helices are stabilized by position-dependent interactions between the lysine side chain and the helix barrel.

Chaperonin-facilitated protein folding: optimization of rate and yield by an iterative annealing mechanism.

Posttranslational amino acid epimerization: enzyme-catalyzed isomerization of amino acid residues in peptide chains.

Interleukin 3 or interleukin 1 abrogates the reconstituting ability of hematopoietic stem cells.

Increased cytosine DNA-methyltransferase activity is target-cell-specific and an early event in lung cancer.

Immunologic basis of transplant-associated arteriosclerosis.

DNA transport by a type II topoisomerase: direct evidence for a two-gate mechanism.

Mapping the protein regions responsible for the functional specificities of the Arabidopsis MADS domain organ-identity proteins.

Homophilic adhesion mediated by the neural cell adhesion molecule involves multiple immunoglobulin domains.

4E-BP1 phosphorylation is mediated by the FRAP-p70s6k pathway and is independent of mitogen-activated protein kinase.

Metabolism of Alzheimer beta-amyloid precursor protein: regulation by protein kinase A in intact cells and in a cell-free system.

Dominant-negative p53 mutations selected in yeast hit cancer hot spots.

Identification of the promoter of the mouse obese gene.

Selective expansion of high- or low-avidity cytotoxic T lymphocytes and efficacy for adoptive immunotherapy.

Ethinylestradiol does not enhance the expression of nitric oxide synthase in bovine endothelial cells but increases the release of bioactive nitric oxide by inhibiting superoxide anion production.

Centripetal cholesterol flux from extrahepatic organs to the liver is independent of the concentration of high density lipoprotein-cholesterol in plasma.

Human semaphorins A(V) and IV reside in the 3p21.3 small cell lung cancer deletion region and demonstrate distinct expression patterns.

Aromatic amino acid transamination and methionine recycling in trypanosomatids.

Group B streptococci escape host immunity by deletion of tandem repeat elements of the alpha C protein.

Foamy virus reverse transcriptase is expressed independently from the Gag protein.

Cell density regulates cellular reversal frequency in Myxococcus xanthus.

Neurocytopathic effects of beta-amyloid-stimulated monocytes: a potential mechanism for central nervous system damage in Alzheimer disease.

Retinal glial cell glutamate transporter is coupled to an anionic conductance.

Calorie restriction lowers body temperature in rhesus monkeys, consistent with a postulated anti-aging mechanism in rodents.

Prolactin increases CD4/CD8 cell ratio in thymus-grafted congenitally athymic nude mice.

The phosphatase activity of carbonic anhydrase III is reversibly regulated by glutathiolation.

Plasmoviruses: nonviral/viral vectors for gene therapy.

Characterization of the transforming activity of p80, a hyperphosphorylated protein in a Ki-1 lymphoma cell line with chromosomal translocation t(2;5).

Molecularly engineered resistance to California serogroup virus replication in mosquito cells and mosquitoes.

Converting cancer genes into killer genes.

Macrophage killing is an essential virulence mechanism of Salmonella typhimurium.

Truncated elongation factor G lacking the G domain promotes translocation of the 3' end but not of the anticodon domain of peptidyl-tRNA.

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Mixed synapses discovered and mapped throughout mammalian spinal cord.

Nerve growth factor in the anterior pituitary: localization in mammotroph cells and cosecretion with prolactin by a dopamine-regulated mechanism.

Nitric oxide synthase content of hypothalamic explants: increase by norepinephrine and inactivated by NO and cGMP.

A general assay for antibody catalysis using acridone as a fluorescent tag.

A rigid trans-spanning dinitrile ligand.

Bond orientational order, molecular motion, and free energy of high-density DNA mesophases.

Characterization of the gene cluster of high-molecular-mass nitrile hydratase (H-NHase) induced by its reaction product in Rhodococcus rhodochrous J1.

Modulation of the transcriptional activity of thyroid hormone receptors by the tumor suppressor p53.

Human plectin: organization of the gene, sequence analysis, and chromosome localization (8q24).

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Differences in the RNA binding sites of iron regulatory proteins and potential target diversity.

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Synthesis and targeted delivery of an azidothymidine homodinucleotide conferring protection to macrophages against retroviral infection.

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Cell cycle-regulated binding of nuclear proteins to elements within a mouse H3.2 histone gene.

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Treatment of a human breast cancer xenograft with an adenovirus vector containing an interferon gene results in rapid regression due to viral oncolysis and gene therapy.

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Targeted DNA recombination in vivo using an adenovirus carrying the cre recombinase gene.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Edelman, G. M. Wang, Y. Krushel, L. A.
Description	Author Affiliation: Wang Y ( Department of Neurobiology, Scripps Research Institute, La Jolla, CA 92037, USA.);
Abstract	Conditional gene expression and gene deletion are important experimental approaches for examining the functions of particular gene products in development and disease. The cre-loxP system from bacteriophage P1 has been used in transgenic animals to induce site-specific DNA recombination leading to gene activation or deletion. To regulate the recombination in a spatiotemporally controlled manner, we constructed a recombinant adenoviral vector, Adv/cre, that contained the cre recombinase gene under regulation of the herpes simplex virus thymidine kinase promoter. The efficacy and target specificity of this vector in mediating loxP-dependent recombination were analyzed in mice that had been genetically engineered to contain loxP sites in their genome. After intravenous injection of the Adv/cre vector into adult animals, the liver and spleen showed the highest infectivity of the adenovirus as well as the highest levels of recombination, whereas other tissues such as kidney, lung, and heart had lower levels of infection and recombination. Only trace levels of recombination were detected in the brain. However, when the Adv/cre vector was injected directly into specific regions of the adult brain, including the cerebral cortex, hippocampus, and cerebellum, recombination was detectable at the injection site. Furthermore, when the Adv/cre vector was injected into the forebrains of neonatal mice, the rearranged toxP locus from recombination could be detected in the injected regions for at least 8 weeks. Taken together, these results demonstrate that the Adv/cre vector expressing a functional cre protein is capable of mediating loxP-dependent recombination in various tissues and the recombined gene locus may in some cases be maintained for an extended period. The use of the adenovirus vector expressing cre combined with localized delivery to specific tissues may provide an efficient means to achieve conditional gene expression or knockout with precise spatiotemporal control.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	9
Volume Number	93
Language	English
Publisher	National Academy of Sciences
Publisher Date	1996-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Brain Metabolism DNA Nucleotidyltransferases Integrases Recombination, Genetic Viral Proteins Adenoviridae Animals Animals, Newborn Bacteriophage P1 Enzymology Genetics Cerebellum Cerebral Cortex DNA, Viral Biosynthesis Genetic Vectors Hippocampus Liver Mice Mice, Transgenic Plasmids Polymerase Chain Reaction Promoter Regions, Genetic Beta-Galactosidase Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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