NDLI: RNA interference blocks gene expression and RNA synthesis from hepatitis C replicons propagated in human liver cells.

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RNA interference blocks gene expression and RNA synthesis from hepatitis C replicons propagated in human liver cells.

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RNA interference blocks gene expression and RNA synthesis from hepatitis C replicons propagated in human liver cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Sabatinos, Sarah Sarangi, Farida Beaulieu, Sylvie Jayasena, Sumedha Khvorova, Anastasia Harris-brandts, Marees Rodrigue-gervais, Ian Gaël Arya, Sudha Wilson, Joyce A. Richardson, Christopher D.
Description	Author Affiliation: Wilson JA ( Ontario Cancer Institute, 620 University Avenue, Suite 706, Toronto, ON, Canada M5G 2C1.);
Abstract	RNA interference represents an exciting new technology that could have therapeutic applications for the treatment of viral infections. Hepatitis C virus (HCV) is a major cause of chronic liver disease and affects >270 million individuals worldwide. The HCV genome is a single-stranded RNA that functions as both a messenger RNA and replication template, making it an attractive target for the study of RNA interference. Double-stranded small interfering RNA (siRNA) molecules designed to target the HCV genome were introduced through electroporation into a human hepatoma cell line (Huh-7) that contained an HCV subgenomic replicon. Two siRNAs dramatically reduced virus-specific protein expression and RNA synthesis to levels that were 90% less than those seen in cells treated with negative control siRNAs. These same siRNAs protected naive Huh-7 cells from challenge with HCV replicon RNA. Treatment of cells with synthetic siRNA was effective >72 h, but the duration of RNA interference could be extended beyond 3 weeks through stable expression of complementary strands of the interfering RNA by using a bicistronic expression vector. These results suggest that a gene-therapeutic approach with siRNA could ultimately be used to treat HCV.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	5
Volume Number	100
Language	English
Publisher	National Academy of Sciences
Publisher Date	2003-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Hepatitis C Metabolism Liver Cytology RNA Interference RNA, Small Interfering Physiology RNA, Viral Genetics RNA Virus Replication Antibodies, Monoclonal Blotting, Northern Blotting, Western Cell Line Electrophoresis, Polyacrylamide Gel Electroporation Genetic Vectors Models, Genetic Mutation Plasmids RNA, Messenger Time Factors Transcription, Genetic Transfection Tumor Cells, Cultured Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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