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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Fumagalli, Laura Dans, Pablo D. Orozco, Modesto Carrascosa, José L. Cuervo, Ana Gomila, Gabriel |
| Description | Author Affiliation: Cuervo A ( Department of Structure of Macromolecules, Centro Nacional de Biotecnologia-Consejo Superior de Investigaciones Cientificas, Campus Cantoblanco, 28049 Madrid, Spain); Dans PD ( Institute for Research in Biomedicine-Barcelona Supercomputing Center Joint Research Program in Computational Biology, Institute for Research in Biomedicine-Barcelona, 08028 Barcelona, Spain); Carrascosa JL ( Department of Structure of Macromolecules, Centro Nacional de Biotecnologia-Consejo Superior de Investigaciones Cientificas, Campus Cantoblanco, 28049 Madrid, Spain); Orozco M ( Institute for Research in Biomedicine-Barcelona Supercomputing Center Joint Research Program in Computational Biology, Institute for Research in Biomedicine-Barcelona, 08028 Barcelona, Spain); Gomila G ( Institut de Bioenginyeria de Catalunya, 08028 Barcelona, Spain); Fumagalli L ( Institut de Bioenginyeria de Catalunya, 08028 Barcelona, Spain); |
| Abstract | The electric polarizability of DNA, represented by the dielectric constant, is a key intrinsic property that modulates DNA interaction with effector proteins. Surprisingly, it has so far remained unknown owing to the lack of experimental tools able to access it. Here, we experimentally resolved it by detecting the ultraweak polarization forces of DNA inside single T7 bacteriophages particles using electrostatic force microscopy. In contrast to the common assumption of low-polarizable behavior like proteins (εr ⠼ 2-4), we found that the DNA dielectric constant is ⠼ 8, considerably higher than the value of ⠼ 3 found for capsid proteins. State-of-the-art molecular dynamic simulations confirm the experimental findings, which result in sensibly decreased DNA interaction free energy than normally predicted by Poisson-Boltzmann methods. Our findings reveal a property at the basis of DNA structure and functions that is needed for realistic theoretical descriptions, and illustrate the synergetic power of scanning probe microscopy and theoretical computation techniques. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 35 |
| Volume Number | 111 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2014-09-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Bacteriophage T7 Genetics Capsid Chemistry DNA, Viral DNA Dielectric Spectroscopy Models, Chemical Cations Metabolism DNA-Binding Proteins Electrochemical Techniques Ligands Microscopy, Atomic Force Nuclear Proteins Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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