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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Koglin, Jason E. Cascio, Duilio Eisenberg, David S. Hattne, Johan Gingery, Mari Brewster, Aaron S. Messerschmidt, Marc M. Rodriguez, Jose Colletier, Jacques-philippe Nass, Karol Sauter, Nicholas K. Doak, R. Bruce Williams, Garth J. Park, Hyun-woo Botha, Sabine Federici, Brian A. Sawaya, Michael R. Goldschmidt, Lukasz Shoeman, Robert L. Schlichting, Ilme Boutet, Sébastien Deponte, Daniel P. |
| Description | Author Affiliation: Sawaya MR ( UCLA-DOE Institute for Genomics and Proteomics, Department of Biological Chemistry, and.); Cascio D ( UCLA-DOE Institute for Genomics and Proteomics, Department of Biological Chemistry, and.); Gingery M ( UCLA-DOE Institute for Genomics and Proteomics, Department of Biological Chemistry, and.); Rodriguez J ( UCLA-DOE Institute for Genomics and Proteomics, Department of Biological Chemistry, and.); Goldschmidt L ( UCLA-DOE Institute for Genomics and Proteomics, Department of Biological Chemistry, and.); Colletier JP ( Université Grenoble Alpes, Centre National de la Recherche Scientifique, and Commissariat à l'Energie Atomique, Institut de Biologie Structurale, F-38044 Grenoble, France); Messerschmidt MM ( Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025); Boutet S ( Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025); Koglin JE ( Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025); Williams GJ ( Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025); Brewster AS ( Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720); Nass K ( Max Planck Institute for Medical Research, 69120 Heidelberg, Germany); Hattne J ( Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720); Botha S ( Max Planck Institute for Medical Research, 69120 Heidelberg, Germany); Doak RB ( Max Planck Institute for Medical Research, 69120 Heidelberg, Germany); Shoeman RL ( Max Planck Institute for Medical Research, 69120 Heidelberg, Germany); DePonte DP ( Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025); Park HW ( Department of Entomology and.); Federici BA ( Department of Entomology and Graduate Program in Cell, Molecular and Developmental Biology, University of California, Riverside, CA 92521.); Sauter NK ( Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720); Schlichting I ( Max Planck Institute for Medical Research, 69120 Heidelberg, Germany); Eisenberg DS ( UCLA-DOE Institute for Genomics and Proteomics, Department of Biological Chemistry, and Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095-1570); |
| Abstract | It has long been known that toxins produced by Bacillus thuringiensis (Bt) are stored in the bacterial cells in crystalline form. Here we describe the structure determination of the Cry3A toxin found naturally crystallized within Bt cells. When whole Bt cells were streamed into an X-ray free-electron laser beam we found that scattering from other cell components did not obscure diffraction from the crystals. The resolution limits of the best diffraction images collected from cells were the same as from isolated crystals. The integrity of the cells at the moment of diffraction is unclear; however, given the short time (â ¼ 5 µs) between exiting the injector to intersecting with the X-ray beam, our result is a 2.9-Å-resolution structure of a crystalline protein as it exists in a living cell. The study suggests that authentic in vivo diffraction studies can produce atomic-level structural information. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 35 |
| Volume Number | 111 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2014-09-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Bacillus Thuringiensis Chemistry Bacterial Proteins Crystallography, X-Ray Endotoxins Hemolysin Proteins Spores, Bacterial Ultrastructure Crystallization Instrumentation Lasers Synchrotrons X-Ray Diffraction Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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