| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Giritch, Anatoli Stahl, Chad H. Paschke, Anne-katrin Hahn, Simone Bettmann, Ulrike Jarczowski, Franziska Gleba, Yuri Schulz, Steve Stephan, Anett Bortesi, Luisa Tusé, Daniel |
| Description |
Author Affiliation: Schulz S ( Nomad Bioscience GmbH, Biozentrum Halle, D-06120 Halle (Saale), Germany); Stephan A ( Nomad Bioscience GmbH, Biozentrum Halle, D-06120 Halle (Saale), Germany); Hahn S ( Nomad Bioscience GmbH, Biozentrum Halle, D-06120 Halle (Saale), Germany); Bortesi L ( Nomad Bioscience GmbH, Biozentrum Halle, D-06120 Halle (Saale), Germany); Jarczowski F ( Icon Genetics GmbH, Biozentrum Halle, D-06120 Halle (Saale), Germany); Bettmann U ( Icon Genetics GmbH, Biozentrum Halle, D-06120 Halle (Saale), Germany); Paschke AK ( Icon Genetics GmbH, Biozentrum Halle, D-06120 Halle (Saale), Germany); Tusé D ( DT/Consulting Group, Sacramento, CA 95818); Stahl CH ( Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20742.); Giritch A ( Nomad Bioscience GmbH, Biozentrum Halle, D-06120 Halle (Saale), Germany); Gleba Y ( Nomad Bioscience GmbH, Biozentrum Halle, D-06120 Halle (Saale), Germany); |
| Abstract |
Enterohemorrhagic Escherichia coli (EHEC) is one of the leading causes of bacterial enteric infections worldwide, causing â ¼100,000 illnesses, 3,000 hospitalizations, and 90 deaths annually in the United States alone. These illnesses have been linked to consumption of contaminated animal products and vegetables. Currently, other than thermal inactivation, there are no effective methods to eliminate pathogenic bacteria in food. Colicins are nonantibiotic antimicrobial proteins, produced by E. coli strains that kill or inhibit the growth of other E. coli strains. Several colicins are highly effective against key EHEC strains. Here we demonstrate very high levels of colicin expression (up to 3 g/kg of fresh biomass) in tobacco and edible plants (spinach and leafy beets) at costs that will allow commercialization. Among the colicins examined, plant-expressed colicin M had the broadest antimicrobial activity against EHEC and complemented the potency of other colicins. A mixture of colicin M and colicin E7 showed very high activity against all major EHEC strains, as defined by the US Department of Agriculture/Food and Drug Administration. Treatments with low (less than 10 mg colicins per L) concentrations reduced the pathogenic bacterial load in broth culture by 2 to over 6 logs depending on the strain. In experiments using meats spiked with E. coli O157:H7, colicins efficiently reduced the population of the pathogen by at least 2 logs. Plant-produced colicins could be effectively used for the broad control of pathogenic E. coli in both plant- and animal-based food products and, in the United States, colicins could be approved using the generally recognized as safe (GRAS) regulatory approval pathway. |
| ISSN |
00278424 |
| e-ISSN |
10916490 |
| Journal |
Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number |
40 |
| Volume Number |
112 |
| Language |
English |
| Publisher |
National Academy of Sciences |
| Publisher Date |
2015-10-01 |
| Publisher Place |
United States |
| Access Restriction |
Open |
| Subject Keyword |
Colicins Metabolism Pharmacology Escherichia Coli O157 Drug Effects Plants, Edible Amino Acid Sequence Animals Beta Vulgaris Genetics Electrophoresis, Polyacrylamide Gel Escherichia Coli Infections Microbiology Growth & Development Food Microbiology Meat Molecular Sequence Data Plants, Genetically Modified Recombinant Proteins Isolation & Purification Spinacia Oleracea Swine Tobacco Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Multidisciplinary |
