NDLI: Free flow electrophoresis of chloroplasts.

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Free flow electrophoresis of chloroplasts.

Content Provider	Semantic Scholar
Author	Dubacq, J. P. Kader, J. C.
Copyright Year	1978
Abstract	Highly purified intact chloroplasts were isolated from spinach (Spinacia oleracea L.) leaves by free flow electrophoresis. Morphological and biochemical studies showed that the fraction enriched in intact chloroplasts has a higher protein to chlorophyll ratio and a higher linolenic acid content than the broken organelles of the other fraction. The intact chloroplasts prepared by electrophoresis retained their capacity for CO(2) fixation. Sodium dodecyl sulfate polyacrylamide gel electrophoresis demonstrated that this fraction was rich in stroma and lamellae proteins. Free flow electrophoresis, which separates organelles and molecules according to their surface charges, is a good technique for producing purified chloroplasts with complete physiological activities.
Starting Page	A20
Ending Page	A20
Page Count	1
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.plantphysiol.org/content/plantphysiol/61/3/465.full.pdf
PubMed reference number	16660315v1
Volume Number	61
Issue Number	3
Journal	Plant physiology
Language	English
Access Restriction	Open
Subject Keyword	Chlorophyll Chloroplasts Gel Electrophoresis (lab technique) Linolenic Acid Organelles Plant Leaves Polyacrylamide Gel Electrophoresis Sodium Spinacia oleracea Stroma polyacrylamide gels
Content Type	Text
Resource Type	Article

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