| Author |
Sun, Meng-xiang Liu, Yuan Shi, Ce Tang, Xingchun Wang, Wei Zhang, Liyao Zhao, Jing Chen, Junyi Li, Xinbo Tian, Shujuan Feng, Xianzhong |
| Description |
Author Affiliation: Liu Y ( Department of Cell and Developmental Biology, College of Life Science, State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, China); Li X ( Department of Cell and Developmental Biology, College of Life Science, State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, China); Zhao J ( Department of Cell and Developmental Biology, College of Life Science, State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, China); Tang X ( Department of Cell and Developmental Biology, College of Life Science, State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, China); Tian S ( Department of Cell and Developmental Biology, College of Life Science, State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, China); Chen J ( Department of Cell and Developmental Biology, College of Life Science, State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, China); Shi C ( Department of Cell and Developmental Biology, College of Life Science, State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, China); Wang W ( Department of Cell and Developmental Biology, College of Life Science, State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, China); Zhang L ( Department of Cell and Developmental Biology, College of Life Science, State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, China); Feng X ( Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Changchun, 130102, China.); Sun MX ( Department of Cell and Developmental Biology, College of Life Science, State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, China); |
| Abstract |
The suspensor is a temporary supporting structure of proembryos. It has been proposed that suspensor cells also possess embryogenic potential, which is suppressed by the embryo as an effect of the embryo-suspensor interaction. However, data to support this hypothesis are not yet available. In this report, using an in vivo living cell laser ablation technique, we show that Arabidopsis suspensor cells can develop into embryos after removing the embryo proper. The embryo proper plays a critical role in maintaining suspensor cell identity. However, this depends on the developmental stage; after the globular embryo stage, the suspensors no longer possess the potential to develop into embryos. We also reveal that hypophysis formation may be essential for embryo differentiation. Furthermore, we show that, after removing the embryo, auxin gradually accumulates in the top suspensor cell where cell division occurs to produce an embryo. Auxin redistribution likely reprograms the fate of the suspensor cell and triggers embryogenesis in suspensor cells. Thus, we provide direct evidence that the embryo suppresses the embryogenic potential of suspensor cells. |
