| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Samji, Tasleem Maher, Leigh Sheridan, Brian S. Benechet, Alexandre P. Lemoine, Francois M. Mempel, Thorsten R. Menon, Manisha Murooka, Thomas T. Khanna, Kamal M. Xu, Daqi |
| Description |
Author Affiliation: Benechet AP ( Department of Immunology, University of Connecticut Health, Farmington, CT 06030); Menon M ( Department of Immunology, University of Connecticut Health, Farmington, CT 06030); Xu D ( Department of Immunology, University of Connecticut Health, Farmington, CT 06030); Samji T ( Department of Immunology, University of Connecticut Health, Farmington, CT 06030); Maher L ( Department of Immunology, University of Connecticut Health, Farmington, CT 06030); Murooka TT ( The Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Boston, MA 02114); Mempel TR ( The Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Boston, MA 02114); Sheridan BS ( Department of Immunology, University of Connecticut Health, Farmington, CT 06030); Lemoine FM ( Centre d'Immunologie et des Maladies Infectieuses de Paris, UMR-S CR7, Université Pierre-et-Marie-Curie, University of Paris 6, Sorbonne Universités, F-75005 Paris, France); Khanna KM ( Department of Immunology, University of Connecticut Health, Farmington, CT 06030); |
| Abstract |
Viral clearance requires effector T-cell egress from the draining lymph node (dLN). The mechanisms that regulate the complex process of effector T-cell egress from the dLN after infection are poorly understood. Here, we visualized endogenous pathogen-specific effector T-cell migration within, and from, the dLN. We used an inducible mouse model with a temporally disrupted sphingosine-1-phosphate receptor-1 (S1PR1) gene specifically in endogenous effector T cells. Early after infection, WT and S1PR1(-/-) effector T cells localized exclusively within the paracortex. This localization in the paracortex by CD8 T cells was followed by intranodal migration by both WT and S1PR1(-/-) T cells to positions adjacent to both cortical and medullary lymphatic sinuses where the T cells exhibited intense probing behavior. However, in contrast to WT, S1PR1(-/-) effector T cells failed to enter the sinuses. We demonstrate that, even when LN retention signals such as CC chemokine receptor 7 (CCR7) are down-regulated, T cell intrinsic S1PR1 is the master regulator of effector T-cell emigration from the dLN. |
| ISSN |
00278424 |
| e-ISSN |
10916490 |
| Journal |
Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number |
8 |
| Volume Number |
113 |
| Language |
English |
| Publisher |
National Academy of Sciences |
| Publisher Date |
2016-02-01 |
| Publisher Place |
United States |
| Access Restriction |
Open |
| Subject Keyword |
Infection Immunology Pathology Lymph Nodes Receptors, Lysosphingolipid T-Lymphocytes Animals Cell Movement Endothelial Cells Lymphocyte Activation Mice Mice, Congenic Mice, Inbred C57BL Mice, Knockout Mice, Transgenic Deficiency Genetics Vesicular Stomatitis Vesicular Stomatitis Indiana Virus Research Support, N.I.H., Extramural Multidisciplinary |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Multidisciplinary |
