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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Laliotis, George P. Vitsa, Alkistis Bizelis, Iosif Charismiadou, Maria A. Rogdakis, Emmanuel |
| Description | Country affiliation: Greece Author Affiliation: Laliotis GP ( Department of Animal Science, Agricultural University of Athens, Greece.) |
| Abstract | Glucose 6-phosphate dehydrogenase (G6PD) plays an important role in a ruminant's metabolism catalyzing the first committed reaction in the pentose phosphate pathway as it provides necessary compounds of NADPH for the synthesis of fatty acids. The cloning of ovine (Ovis aries) G6PD gene revealed the presence of two cDNA transcripts (oG6PD(A) and oG6PD(B)), with oG6PD(B) being a product of alternative splicing and with no similarity to any other previously reported G6PD transcript. Here, we attempt to study the effect of energy balance in ovine G6PD transcript expression, trying simultaneously to find out any potential physiological role of the oG6PD(B) transcript. Changes of energy balance that lead to synergistic changes in the expression of both transcripts, but in opposite directions and not in a proportional way. Negative energy balance favours the presence of the oG6PD(B) transcript leading to a significant increase of its expression, compared to oG6PD(A) expression (P<0.05). In contrast, positive energy balance leads to a significant increase of oG6PD(A) compared to oG6PD(B) expression (P<0.05). In either condition oG6PD(B) expression is unchanged. Regression analysis showed that there is an energy balance threshold where the expression of both transcripts shows no change. |
| File Format | HTM / HTML |
| ISSN | 1744117X |
| Issue Number | 2 |
| Volume Number | 5 |
| e-ISSN | 18780407 |
| Journal | Comparative Biochemistry and Physiology Part D: Genomics and Proteomics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-06-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Genetics Discipline Biochemistry Energy Intake Gene Expression Regulation Glucosephosphate Dehydrogenase Genetics Sheep, Domestic Animals Gene Expression Profiling Metabolism Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Physiology Molecular Biology Biochemistry Aquatic Science Animal Science and Zoology |
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