NDLI: Energy balance-dependent regulation of ovine glucose 6-phosphate dehydrogenase protein isoform expression

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Energy balance-dependent regulation of ovine glucose 6-phosphate dehydrogenase protein isoform expression

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Year: 2013, Volume: 2

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Energy balance-dependent regulation of ovine glucose 6-phosphate dehydrogenase protein isoform expression

Content Provider	PubMed Central
Author	Triantaphyllopoulos, Kostas A. Laliotis, George P. Bizelis, Iosif A.
Copyright Year	2014
Abstract	G6PDH is the rate-limiting enzyme of the pentose phosphate pathway and one of the principal source of NADPH, a major cellular reductant. Importantly, in ruminant's metabolism the aforementioned NADPH provided, is utilized for de novo fatty acid synthesis. Previous work of cloning the ovine (Ovis aries) og6pdh gene has revealed the presence of two cDNA transcripts (og6pda and og6pdb), og6pdb being a product of alternative splicing not similar to any other previously reported.1 In the current study the effect of energy balance in the ovine G6PDH protein expression was investigated, shedding light on the biochemical features and potential physiological role of the oG6PDB isoform. Changes in energy balance leads to protein expression changes in both transcripts, to the opposite direction and not in a proportional way. Negative energy balance was not in favor of the presence of any particular isoform, while both protein expression levels were not significantly different (P > 0.05). In contrast, at the transition point from negative to positive and on the positive energy balance, there is a significant increase of oG6PDA compared with oG6PDB protein expression (P < 0.001). Both oG6PDH protein isoforms changed significantly toward the positive energy balance. oG6PDA is escalating, while oG6PDB is falling, under the same stimulus (positive energy balance alteration). This change is also positively associated with increasing levels in enzyme activity, 4 weeks post-weaning in ewes’ adipose tissue. Furthermore, regression analysis clearly demonstrated the linear correlation of both proteins in response to the WPW, while energy balance, enzyme activity, and oG6PDA relative protein expression follow the same escalating trend; in contrast, oG6PDB relative protein expression falls in time, similar to both transcripts accumulation pattern, as reported previously.2
Related Links	http://dx.doi.org/10.4161/adip.26437
Ending Page	38
Page Count	9
Starting Page	30
File Format	PDF
ISSN	21623945
e-ISSN	2162397X
Journal	Adipocyte
Issue Number	1
Volume Number	3
Language	English
Publisher	Landes Bioscience
Publisher Date	2014-01-28
Access Restriction	Open
Rights Holder	Landes Bioscience
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology

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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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Energy balance-dependent regulation of ovine glucose 6-phosphate dehydrogenase protein isoform expression

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