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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Loppion, Géraldine Lavigne, Régis Pineau, Charles Auvray, Pierrïck Sourdaine, Pascal |
| Description | Country affiliation: France Author Affiliation: Loppion G ( UMR M Ifremer, Physiologie et Ecophysiologie des Mollusques Marins, IFR ICORE, Université de Caen Basse-Normandie, France.) |
| Abstract | In the dogfish (Scyliorhinus canicula L.) the testicular germinative zone (GZ), composed of large isolated spermatogonia surrounded by elongating pre-Sertoli cells, is located between the albuginea and the ventrolateral intratesticular vessel. During the spermatogenic wave, cysts radiate in maturational order forming distinct testicular zones. In this study, soluble proteins of the GZ and of the zone containing cysts with spermatocytes were separated by two-dimensional electrophoresis. Gel images were matched and then evaluated for GZ-specific proteins. From the1400 protein spots identified, 680 were found to be apparently specific to this zone. Using MALDI-TOF/TOF mass spectrometry, de novo sequences were obtained for 33 proteins out of the 169 selected for identification by mass spectrometry, but only 16 of these 169 proteins were identified. One of them, proteasome subunit alpha-6, was analyzed further by immunohistochemistry. This study demonstrates the utility of the dogfish as a model for proteome analysis of the spermatogonial stem cell niche, even if it remains restricted by the lack of genomic data available on Elasmobranchs. |
| File Format | HTM / HTML |
| ISSN | 1744117X |
| Issue Number | 2 |
| Volume Number | 5 |
| e-ISSN | 18780407 |
| Journal | Comparative Biochemistry and Physiology Part D: Genomics and Proteomics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-06-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Genetics Discipline Biochemistry Dogfish Metabolism Proteome Spermatogonia Stem Cells Animals Blotting, Western Immunohistochemistry Male Mass Spectrometry Analysis Spermatogenesis Testis Cytology Ultrastructure Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Physiology Molecular Biology Biochemistry Aquatic Science Animal Science and Zoology |
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