NDLI: Differential protein expression profile in the liver of pikeperch (Sander lucioperca) larvae fed with increasing levels of phospholipids.

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Differential protein expression profile in the liver of pikeperch (Sander lucioperca) larvae fed with increasing levels of phospholipids.

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Differential protein expression profile in the liver of pikeperch (Sander lucioperca) larvae fed with increasing levels of phospholipids.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hamza, Neïla Silvestre, Frédéric Mhetli, Mohamed Khemis, Ines Ben Dieu, Marc Raes, Martine Cahu, Chantal Kestemont, Patrick
Description	Author Affiliation: Hamza N ( Institut National des Sciences et Technologies de la Mer, Salammbo, Tunisia. hamza.neila@gmail.com )
Abstract	A comparative proteomic approach was used to assess the protein expression profile in the liver of 34days old pikeperch larvae fed from day 10 post hatching, with three isoproteic and isolipidic formulated diets varying by their phospholipid (PL) contents (% dry diet weight): 1.4% (PL1), 4.7% (PL5) and 9.5% (PL9). Using 2D-DIGE minimal labelling of liver extracts, we were able to show 56 protein spots with a differential intensity (p<0.05) depending on the dietary PL content. Among these spots, 11 proteins were unambiguously identified using nanoLC-MS/MS tandem mass spectrometry. In the PL9 larvae, our results indicate that the glycolytic pathway could be down-regulated due to the under-expression of the fructose biphosphate aldolase B and the phosphoglucomutase 1. Meanwhile, propionyl coenzyme A carboxylase (a gluconeogenic enzyme) was under-expressed. In addition, another gluconeogenic and lipogenic enzyme, pyruvate carboxylase, was identified in 3 different spots as being under-expressed in fish fed with the intermediate PL level (PL5). A high PL content increased the expression of sarcosine dehydrogenase, an enzyme involved in methionine metabolism, along with vinculin, a structural protein. Moreover, several stress proteins (glutathione S-transferase M, glucose regulated protein 75 and peroxiredoxin-1) were modulated in response to the dietary PL level and fatty acid composition. In the larvae fed with the lowest dietary PL content (PL1), over-expression of both GSTM and GRP75 might indicate a cellular stress in this experimental treatment, while the under-expression of Prx1 might indicate a lower defence against oxidative stress. In conclusion, this nutriproteomic approach showed significant modifications of protein expression in the liver of pikeperch larvae fed different PL contents, highlighting the importance of these nutrients and their influence on metabolism processes and on stress response.
File Format	HTM / HTML
ISSN	1744117X
Issue Number	2
Volume Number	5
e-ISSN	18780407
Journal	Comparative Biochemistry and Physiology Part D: Genomics and Proteomics
Language	English
Publisher	Elsevier
Publisher Date	2010-06-01
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Genetics Discipline Biochemistry Dietary Fats Metabolism Fish Proteins Genetics Gene Expression Profiling Larva Liver Perciformes Phospholipids Animals Gene Expression Regulation, Developmental Growth & Development Proteomics Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Physiology Molecular Biology Biochemistry Aquatic Science Animal Science and Zoology

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